Posgrade in Experimental Biology, Division of Health and Biological Sciences, Metropolitan Autonomous University of Iztapalapa, Doctores, Mexico DF, Mexico; Department of Health Sciences, Division of Health and Biological Sciences, Metropolitan Autonomous University of Iztapalapa, Doctores, Mexico DF, Mexico; Unit of Medical Investigation in Biochemistry, Hospital of Specialties, CMNSXXI, Mexican Institute of Social Security, Doctores, Mexico DF, Mexico.
J Pharm Pharmacol. 2013 Oct;65(10):1563-76. doi: 10.1111/jphp.12119. Epub 2013 Aug 5.
Cucurbita ficifolia (characterised by its D chiro inositol (DCI) content) and of synthetic DCI on the redox state, mRNA expression and secretions of proinflammatory cytokines. Additionally, we evaluated the insulin-mimetic action of both treatments by assessing protein kinase B (PKB) activation in 3T3-L1 adipocytes.
Adipocytes were treated with C. ficifolia and synthetic DCI. The redox state was determined by spectrophotometry as changes in the reduced glutathione/oxidised glutathione (GSH/GSSG) ratio, glutathione peroxidase and glutathione reductase activities; H2 O2 levels were measured by flow cytometry. The mRNA expression and the protein level of cytokines were determinate by real-time reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The activation of PKB activation was detected by Western blot.
C. ficifolia extract and synthetic DCI reduced oxidative stress by decreased H2 O2 levels, increased glutathione peroxidase activity and changes in the GSH/GSSG ratio. Furthermore, DCI decreased the mRNA expression and secretion of tumour necrosis factor-α, interleukin 6 (IL-6) and resistin, while C. ficifolia reduced protein levels of resistin and increased IL-6 levels. Only DCI demonstrated insulin-mimetic action.
The antioxidant and anti-inflammatory effects of C. ficifolia extract can be explained in part by its DCI content, which modulates the GSH/GSSG ratio and contributes to a reduced proinflammatory state. C. ficifolia and DCI treatments may reduce the disturbances caused by oxidative stress. Additionally, DCI may improve insulin sensitivity through its insulin-mimetic effects.
研究葫芦科苦瓜（以其 D 手性肌醇（DCI）含量为特征）和合成 DCI 对氧化还原状态、促炎细胞因子的 mRNA 表达和分泌的影响。此外，我们还通过评估 3T3-L1 脂肪细胞中蛋白激酶 B（PKB）的激活来评估这两种治疗方法的胰岛素模拟作用。
用苦瓜和合成 DCI 处理脂肪细胞。通过分光光度法测定氧化还原状态，即还原型谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）比值、谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性的变化；通过流式细胞术测定 H2O2 水平。通过实时逆转录聚合酶链反应和酶联免疫吸附试验分别测定细胞因子的 mRNA 表达和蛋白水平。通过 Western blot 检测 PKB 激活。
苦瓜提取物和合成 DCI 通过降低 H2O2 水平、增加谷胱甘肽过氧化物酶活性和改变 GSH/GSSG 比值来减轻氧化应激。此外，DCI 降低了肿瘤坏死因子-α、白细胞介素 6（IL-6）和抵抗素的 mRNA 表达和分泌，而苦瓜降低了抵抗素的蛋白水平并增加了 IL-6 水平。只有 DCI 具有胰岛素模拟作用。
苦瓜提取物的抗氧化和抗炎作用部分可以用其 DCI 含量来解释，DCI 可以调节 GSH/GSSG 比值，有助于减轻促炎状态。苦瓜和 DCI 处理可能会减轻氧化应激引起的紊乱。此外，DCI 可能通过其胰岛素模拟作用改善胰岛素敏感性。
