利用果蝇幼虫研究表皮伤口愈合与炎症。
Using Drosophila larvae to study epidermal wound closure and inflammation.
作者信息
Burra Sirisha, Wang Yan, Brock Amanda R, Galko Michael J
机构信息
Department of Biochemistry and Molecular Biology, The University of Texas Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
出版信息
Methods Mol Biol. 2013;1037:449-61. doi: 10.1007/978-1-62703-505-7_26.
Abstract
This methods chapter describes two methods for creating epithelial wounds in Drosophila larvae: pinch and puncture wounding. It also covers protocols for visualizing epithelial wounds, either in a dissected whole mount preparation or, using transgenic reporter larvae, in a live whole mount preparation. Finally, useful transgenic lines for live genetic screening of genes required for wound closure or inflammation are described.
摘要
本章方法描述了在果蝇幼虫中创建上皮伤口的两种方法
捏伤和穿刺伤。它还涵盖了在上皮伤口可视化的方案，既可以在解剖的整装标本制备中进行，也可以使用转基因报告幼虫在活的整装标本制备中进行。最后，描述了用于伤口闭合或炎症所需基因的活体遗传筛选的有用转基因品系。
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