Tetracore, Inc., 9901 Belward Campus Drive, Suite 300, Rockville, MD 20850, USA.
Chem Soc Rev. 2013 Nov 21;42(22):8769-98. doi: 10.1039/c3cs60144g.
A nucleic acid thermal cycler is considered to be portable if it is under ten pounds, easily carried by one individual, and battery powered. Nucleic acid amplification includes both polymerase chain reaction (e.g. PCR, RT-PCR) and isothermal amplification (e.g. RPA, HDA, LAMP, NASBA, RCA, ICAN, SMART, SDA). There are valuable applications for portable nucleic acid thermocyclers in fields that include clinical diagnostics, biothreat detection, and veterinary testing. A system that is portable allows for the distributed detection of targets at the point of care and a reduction of the time from sample to answer. The designer of a portable nucleic acid thermocycler must carefully consider both thermal control and the detection of amplification. In addition to thermal control and detection, the designer may consider the integration of a sample preparation subsystem with the nucleic acid thermocycler. There are a variety of technologies that can achieve accurate thermal control and the detection of nucleic acid amplification. Important evaluation criteria for each technology include maturity, power requirements, cost, sensitivity, speed, and manufacturability. Ultimately the needs of a particular market will lead to user requirements that drive the decision between available technologies.
如果一台核酸热循环仪的重量在 10 磅以下，能够被一个人轻松携带，并且使用电池供电，那么就可以认为它是便携式的。核酸扩增包括聚合酶链反应（例如 PCR、RT-PCR）和等温扩增（例如 RPA、HDA、LAMP、NASBA、RCA、ICAN、SMART、SDA）。在临床诊断、生物威胁检测和兽医检测等领域，便携式核酸热循环仪具有重要的应用价值。便携式系统可实现目标在护理点的分布式检测，并减少从样本到答案的时间。便携式核酸热循环仪的设计者必须仔细考虑热控制和扩增检测。除了热控制和检测之外，设计者还可以考虑将样品制备子系统与核酸热循环仪集成在一起。有多种技术可以实现精确的热控制和核酸扩增检测。每种技术的重要评估标准包括成熟度、功率要求、成本、灵敏度、速度和可制造性。最终，特定市场的需求将导致用户需求，从而推动在可用技术之间做出决策。
