Section for Pathology, The Gade Institute, University of Bergen, Bergen, Norway.
1] Section for Pathology, The Gade Institute, University of Bergen, Bergen, Norway [2] Department of Dermatology, Haukeland University Hospital, Bergen, Norway.
Mod Pathol. 2014 Mar;27(3):396-401. doi: 10.1038/modpathol.2013.151. Epub 2013 Sep 13.
The intermediate filament nestin, a neural stem-cell marker, is reported to be expressed more strongly in melanomas compared with benign melanocytic lesions, and increasingly expressed in advanced melanoma stages. However, the prognostic impact of nestin on melanoma has not been well elucidated. The aim of the present study was to evaluate the prognostic influence of nestin expression in cutaneous melanoma in comparison with standard clinico-pathologic variables. In a large series of nodular cutaneous melanoma (n=348), nestin expression was assessed by immunohistochemistry using tissue microarray (TMA) sections. For comparison, nestin staining in corresponding metastases as well as in superficial spreading melanomas and benign nevi was also examined. Nestin was expressed to varying degrees in a majority of nodular melanomas (92%), and was significantly associated with increased tumor thickness, high mitotic count, and the presence of ulceration and tumor necrosis. Also, expression was stronger in the nodular type than in superficial spreading melanomas and benign nevi, but without significant difference when compared with matched metastases from the former. Importantly, strong expression of nestin was significantly associated with reduced survival in multivariate analysis. In conclusion, increased nestin expression was associated with aggressive melanoma features, with independent prognostic impact on multivariate survival analysis when compared with clinico-pathologic factors.
中间丝巢蛋白是一种神经干细胞标志物，据报道，它在黑色素瘤中的表达比良性黑色素细胞病变更强，并且在晚期黑色素瘤中表达逐渐增强。然而，巢蛋白对黑色素瘤的预后影响尚未得到很好的阐明。本研究的目的是评估巢蛋白在皮肤黑色素瘤中的表达对预后的影响，并与标准临床病理变量进行比较。在一项大型结节性皮肤黑色素瘤（n=348）研究中，使用组织微阵列（TMA）切片通过免疫组织化学评估巢蛋白的表达。为了进行比较，还检查了相应转移灶、浅表扩散性黑色素瘤和良性痣中的巢蛋白染色。巢蛋白在大多数结节性黑色素瘤（92%）中呈不同程度表达，与肿瘤厚度增加、有丝分裂计数高、溃疡和肿瘤坏死有关。此外，在结节型黑色素瘤中的表达强于浅表扩散性黑色素瘤和良性痣，但与前者的匹配转移灶相比无显著差异。重要的是，巢蛋白的强表达与多因素生存分析中的生存时间缩短显著相关。总之，巢蛋白表达增加与侵袭性黑色素瘤特征相关，与临床病理因素相比，在多因素生存分析中具有独立的预后影响。
