Department of Applied Microbiology and Biotechnology , Dr. H. S. Gour Vishwavidyalaya, Sagar , India.
Braz J Microbiol. 2010 Jan;41(1):196-200. doi: 10.1590/S1517-838220100001000028. Epub 2010 Mar 1.
Feather waste is generated in large amounts as a by-product of commercial poultry processing. This residue is almost pure keratin, which is not easily degradable by common proteolytic enzymes. Eight strains of Bacillus, isolated from decomposing feathers were tested for the hydrolysis of feather wastes in the laboratory. Among these strains, Bacillus cereus KB043 was the best feather degrading organism when grown on basal medium containing 1% hen feather as sole source of carbon and nitrogen. It caused 78.16 ± 0.4 % degradation with a significant release of soluble protein (1206.15 ± 14.7 µg mL(-1)) and cysteine (20.63 ± 0.4 µg mL(-1)) in the cultivation fluid. The strain also showed the highest level of keratinase activity (39.10 ± 0.4 U mL(-1)). These data indicates that the Bacillus cereus KB043 could be useful in management of poultry wastes.
羽毛废料是商业家禽加工的大量副产品。这种残渣几乎是纯角蛋白,不易被常见的蛋白酶降解。从分解羽毛中分离出的 8 株芽孢杆菌在实验室中测试了水解羽毛废料的能力。在这些菌株中,当在含有 1%鸡毛作为唯一碳源和氮源的基础培养基上生长时,蜡状芽孢杆菌 KB043 是最好的羽毛降解菌。它导致 78.16±0.4%的降解,同时在培养液中释放出显著量的可溶性蛋白(1206.15±14.7µg mL(-1))和半胱氨酸(20.63±0.4µg mL(-1))。该菌株还表现出最高水平的角蛋白酶活性(39.10±0.4 U mL(-1))。这些数据表明,蜡状芽孢杆菌 KB043 可能有助于家禽废物的管理。