Department of Biotechnology, Ming Chuan University , No. 5, De-Ming Rd., Gui-Shan Township, Taoyuan County 333 , Taiwan.
Braz J Microbiol. 2010 Oct;41(4):916-21. doi: 10.1590/S1517-838220100004000010. Epub 2010 Dec 1.
The objective of this study was to isolate, characterize, and identify lactic acid bacteria (LAB) from ripe mulberries collected in Taiwan. Ripe mulberry samples were collected at five mulberry farms, located in different counties of Taiwan. Eighty-eight acid-producing cultures were isolated from these samples, and isolates were divided into classes first by phenotype, then into groups by restriction fragment length polymorphism (RFLP) analysis and sequencing of 16S ribosomal DNA (rDNA). Phenotypic and biochemical characteristics led to identification of four bacterial groups (A to D). Weissella cibaria was the most abundant type of LAB distributed in four mulberry farms, and Lactobacillus plantarum was the most abundant LAB found in the remaining farm. Ten W. cibaria and one Lactococcus lactis subsp. lactis isolate produced bacteriocins against the indicator strain Lactobacillus sakei JCM 1157(T). These results suggest that various LAB are distributed in ripe mulberries and W. cibaria was the most abundant LAB found in this study.
本研究旨在从台湾采集的成熟桑椹中分离、鉴定和鉴定乳酸菌（LAB）。从这 5 个桑椹农场采集了 88 个产酸培养物，并通过表型首先将分离物分为类，然后通过限制性片段长度多态性（RFLP）分析和 16S 核糖体 DNA（rDNA）测序将其分为组。表型和生化特征导致鉴定出四个细菌群（A 至 D）。韦氏乳杆菌是分布在 4 个桑椹农场的最丰富的 LAB 类型，而植物乳杆菌是在剩余的农场中发现的最丰富的 LAB。10 个韦氏乳杆菌和 1 个乳球菌乳亚种乳亚种分离株对指示菌株乳杆菌 sakei JCM 1157（T）产生细菌素。这些结果表明，各种 LAB 分布在成熟的桑椹中，而韦氏乳杆菌是本研究中发现的最丰富的 LAB。
