Universidade Paranaense, Laboratório de Biologia Molecular, Mascarenhas de Moraes , Umuarama, PR , Brasil.
Braz J Microbiol. 2011 Apr;42(2):616-23. doi: 10.1590/S1517-838220110002000026. Epub 2011 Jun 1.
This study aimed to verify the biological efficiency and production flushes of Agaricus blazei strains on different casing layers during 90 cultivation days. Four casing layers were used: mixture of subsoil and charcoal (VCS), lime schist (LSC), São Paulo peat (SPP) and Santa Catarina peat (SCP); and two genetically distant A. blazei strains. The fungus was grown in composted substratum and, after total colonization, a pasteurized casing layer was added over the substratum, and fructification was induced. Mushrooms were picked up daily when the basidiocarp veil was stretched, but before the lamella were exposed. The biological efficiency (BE) was determined by the fresh basidiocarp mass divided by the substratum dry mass, expressed in percentage. The production flushes were also determined over time production. The BE and production flushes during 90 days were affected by the strains as well as by the casing layers. The ABL26 and LSC produced the best BE of 60.4%. Although VCS is the most used casing layer in Brazil, it is inferior to other casing layers, for all strains, throughout cultivation time. The strain, not the casing layer, is responsible for eventual variations of the average mushroom mass. In average, circa 50% of the mushroom production occurs around the first month, 30% in the second month, and 20% in third month. The casing layer water management depends on the casing layer type and the strain. Production flush responds better to water reposition, mainly with ABL26, and better porosity to LSC and SCP casing layers.
本研究旨在验证不同覆土层在 90 天栽培期内对巴西蘑菇菌株的生物效率和产量的影响。使用了四种覆土层:表土和木炭的混合物(VCS)、石灰片岩(LSC)、圣保罗泥炭(SPP)和圣卡塔琳娜泥炭(SCP);以及两个遗传上不同的巴西蘑菇菌株。真菌在堆肥基质中生长,在完全定植后,在基质上添加经过巴氏消毒的覆土层,诱导出菇。当菌盖开始拉伸,但菌褶尚未暴露时,每天都会采摘蘑菇。生物效率(BE)通过新鲜菌盖质量除以基质干质量来确定,以百分比表示。随着时间的推移,还确定了产量高峰。90 天内的 BE 和产量高峰受到菌株和覆土层的影响。ABL26 和 LSC 产生了最佳的 BE,为 60.4%。尽管 VCS 是巴西最常用的覆土层,但它不如其他覆土层,对于所有菌株,在整个栽培过程中都表现较差。是菌株而不是覆土层决定了蘑菇平均质量的最终变化。平均而言,大约 50%的蘑菇产量发生在第一个月左右,30%在第二个月,20%在第三个月。覆土层的水分管理取决于覆土层类型和菌株。产量高峰对水分重新定位的响应更好,特别是 ABL26,对 LSC 和 SCP 覆土层的透气性响应更好。