Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University , Suwon 449-701 , Korea.
Braz J Microbiol. 2011 Apr;42(2):794-806. doi: 10.1590/S1517-838220110002000048. Epub 2011 Jun 1.
Cylindrocarpon destructans isolated from ginseng field was found to produce pectinolytic enzymes. A Taguchi's orthogonal array experimental design was applied to optimize the preliminary production of polygalacturonase (PG) and pectin lyase (PL) using submerged culture condition. This method was applied to evaluate the significant parameters for the production of enzymes. The process variables were pH, pectin concentration, incubation time and temperature. Optimization of process parameters resulted in high levels of enzyme (PG and PL) production after ten days of incubation at a pH of 5.0 at 25°C in the presence of 1.5% pectin. Among different nitrogen sources, urea and peptone showed high production of PG and PL, respectively. The enzyme production and mycelial growth seems to have direct influence on the culture conditions; therefore, at stationary state high enzyme production and mycelial growth were obtained than agitation state. Along with this, optimization of enzyme activity was also determined using various physiological parameters like, temperature, incubation time and pH. Taguchi's data was also analyzed using one step ANOVA statistical method.
从人参田地中分离到的旋孢腔菌被发现能产生果胶酶。采用田口正交数组实验设计，对利用深层发酵条件初步生产聚半乳糖醛酸酶（PG）和果胶裂解酶（PL）进行了优化。该方法用于评估对酶生产有显著影响的参数。过程变量为 pH 值、果胶浓度、培养时间和温度。经过十天的培养，在 pH 值为 5.0、25°C、果胶浓度为 1.5%的条件下，对工艺参数进行优化，可得到高水平的酶（PG 和 PL）产量。在不同的氮源中，尿素和蛋白胨分别显示出高的 PG 和 PL 生产能力。酶的产生和菌丝体的生长似乎对培养条件有直接影响；因此，在静止状态下可获得比搅拌状态更高的酶产量和菌丝体生长。同时，还使用各种生理参数（如温度、培养时间和 pH 值）对酶活性的优化进行了测定。田口数据也使用单步方差分析统计方法进行了分析。
