Department of Applied Microbiology and Biotechnology, Dr. Hari Singh Gour University , Sagar, M.P. 470003 , India.
Braz J Microbiol. 2012 Jan;43(1):62-9. doi: 10.1590/S1517-83822012000100007. Epub 2012 Jun 1.
Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL(-1)) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL(-1)). The yeast showed good growth on a simple medium containing dahlia extract (20% w/v) and yeast extract (2%w/v) as carbon and nitrogen source respectively, in 96 h. at 28°C and 120 rpm. Lowest inulinase yield (4.8 nkat mL(-1)) was seen in the medium containing glucose as C-source. Although varied inulinase levels were noticed on different C- sources, Inulinase: Sucrase (I/S) ratios were noticed to be similar. Among various protein sources tested, yeast extract was found to be the best source followed by beef extract (17.9 nkat mL(-1)) and peptone (13.8 nkat mL(-1)). The enzyme was optimally active at pH (4.0) and 50°C. TLC analysis of end product revealed that inulinase hydrolyzed inulin exclusively into fructose. Results suggest that the dahlia extract induced exoinulinase synthesis in Kluyveromyces marxianus and can be utilized as a potential substrate for inulinase production.
各种碳源被评估用于酵母 Kluyveromyces marxianus MTCC 3995 生产菊粉酶。以大丽花提取物（25.3 nkat mL(-1))作为碳源时，观察到最高的菊粉酶活性。该酶活性比含有纯菊苣菊粉（17.8 nkat mL(-1))的培养基中观察到的活性高 1.4 倍。该酵母在含有大丽花提取物（20%w/v）和酵母提取物（2%w/v）的简单培养基中表现出良好的生长，在 28°C 和 120 rpm 下 96 h 后即可生长。以葡萄糖作为 C 源时，观察到最低的菊粉酶产量（4.8 nkat mL(-1))。虽然在不同的 C 源上观察到不同的菊粉酶水平，但观察到菊粉酶：蔗糖酶（I/S）比值相似。在所测试的各种蛋白质源中，发现酵母提取物是最佳来源，其次是牛肉提取物（17.9 nkat mL(-1))和蛋白胨（13.8 nkat mL(-1))。该酶在 pH（4.0)和 50°C 时活性最佳。末端产物的 TLC 分析表明，菊粉酶将菊粉专一地水解成果糖。结果表明，大丽花提取物诱导 Kluyveromyces marxianus 合成外切菊粉酶，可作为菊粉酶生产的潜在底物。
