Singh R S, Bhermi H K
Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147 002, Punjab, India.
Bioresour Technol. 2008 Oct;99(15):7418-23. doi: 10.1016/j.biortech.2008.01.004. Epub 2008 Feb 14.
Root tubers of Asparagus officinalis were used as a source of raw inulin for the production of exoinulinase (EC 3.2.1.7) from Kluyveromyces marxianus YS-1. Root extract prepared at 10kg/cm2 pressure for 10min showed maximum inulinase production. Medium components and process parameters were standardized to improve the enzyme production. Inulinase yield of 40.2IU/mL in a medium containing raw inulin (3.5%), beef extract (2%), SDS (0.001%), Mn2+ (2.0mM), Mg2+ (1.5mM), Co2+ (2mM) and pH 6.5 has been obtained under agitation (150rpm) after 60h of incubation at 30 degrees C at shake flask level. After optimization, the enzyme production was 4.8 times more than the basal medium. To test the feasibility of raw inulin from A. officinalis for the production of inulinase, trials were also made in a bioreactor (1.5L). Inulinase activity of 50.2IU/mL was obtained from raw inulin (4.0%) under agitation (200rpm) and aeration (0.75vvm) at 30 degrees C after 60h of fermentation. Inulinase yield in bioreactor was almost six times higher than the basal medium used initially in shake flask.
芦笋的块根被用作生产马克斯克鲁维酵母YS-1胞外菊粉酶（EC 3.2.1.7）的粗菊粉来源。在10kg/cm2压力下制备10分钟的根提取物显示出最大的菊粉酶产量。对培养基成分和工艺参数进行了标准化以提高酶产量。在含有粗菊粉（3.5%）、牛肉提取物（2%）、SDS（0.001%）、Mn2+（2.0mM）、Mg2+（1.5mM）、Co2+（2mM）且pH为6.5的培养基中，于30℃摇瓶培养60小时，在搅拌（150rpm）条件下获得了40.2IU/mL的菊粉酶产量。优化后，酶产量比基础培养基提高了4.8倍。为测试芦笋粗菊粉用于生产菊粉酶的可行性，还在1.5L生物反应器中进行了试验。在30℃下发酵60小时后，在搅拌（200rpm）和通气（0.75vvm）条件下，从4.0%的粗菊粉中获得了50.2IU/mL的菊粉酶活性。生物反应器中的菊粉酶产量几乎比最初在摇瓶中使用的基础培养基高六倍。
