The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi 214122 , People's Republic of China ; Fishery Extension Station of Yingzhou District , 28 Yingzhou Road, Fuyang 236000, Anhui Province , People's Republic of China.
Braz J Microbiol. 2012 Jan;43(1):177-86. doi: 10.1590/S1517-838220120001000019. Epub 2012 Jun 1.
The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.
本研究报道了一种从土壤中分离出的新型细菌菌株产几丁质酶的统计中值优化，该菌株被命名为解几丁质菌 SYBC-H1。应用包含 Plackett-Burman 和响应面法（RSM）的序贯统计方法来提高几丁质酶的发酵生产，其中菊粉首先被用作有效的碳源。结果，在优化的培养基中获得了最大的几丁质酶活性为 5.17 U/mL，比基础培养基中的活性高 15.5 倍。在优化的营养水平下进行了三次验证实验，结果表明它与预测值吻合良好。
