Laboratório de Bioquímica de Alimentos, Departamento de Ciências de Alimentos, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas , Campinas, SP , Brasil.
Braz J Microbiol. 2009 Jul;40(3):623-30. doi: 10.1590/S1517-838220090003000026. Epub 2009 Sep 1.
This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25°C and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25°C and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts.
本研究涉及来自纤维单胞菌菌株 191 的细胞外几丁质酶的生产、纯化和应用。在摇瓶中，在 25°C 和 200rpm 下培养 72 小时后，几丁质酶的最大产量为 6.9U/mL。在 5L 发酵罐中，通气量为 1.5vvm 时，在 25°C 和 200rpm 下发酵 168 小时后，最高产量为 4.19U/mL，而通气量为 3vvm 时，发酵 144 小时后产量为 4.38U/mL。几丁质酶（61kDa）通过 Sepharose CL-4B 200 凝胶过滤纯化约 6.65 倍，产率为 46.61%。纯化的酶能够裂解一些真菌的细胞壁并形成原生质体。
