Key Laboratory of Forest Protection, College of Forestry and Biotechnology, Zhejiang Agricultural and Forestry University , Lin'an 311300 , People's Republic of China.
Braz J Microbiol. 2012 Jan;43(1):325-31. doi: 10.1590/S1517-838220120001000038. Epub 2012 Jun 1.
Pandora neoaphidis overwintering had been investigated by monitoring its prevalence in Myzus persicae populations in open fields. Cabbage plants in field plots were weekly taken after mycosis initiation, to count and examine the living and dead aphids infected by P. neoaphidis . Based on the field data, infection levels ( I) varied with field temperature (T), relative humidity (RH) and aphid count (numbers of living aphids per plant, N) over days ( D), fitting well to the modified logistic equation I =0.91/[1+exp(8.5+(2.0 H T H RH-20.2 NI 0) D)] ( r (2)=0.897), where H T indicated daily hours of low temperature (<4°C), H RH daily hours of high air humidity (>90% RH) and I 0 primary infection level. The model demonstrated the abiotic and biotic factors influencing P. neoaphidis mycosis development in winter, and also verifies the fungal overwintering by infecting available host aphids without a resting stage. Ultimately, P. neoaphidis mycosis reduced 81.4% of aphid populations, presenting great potential for biocontrol.
已通过监测田间烟粉虱种群中其流行率的方法对球孢白僵菌的越冬情况进行了研究。在发病后，每周从田间小区中取出白菜植株，以统计和检查感染球孢白僵菌的活死蚜虫。基于田间数据，感染水平（I）随田间温度（T）、相对湿度（RH）和蚜虫计数（每株活蚜虫数量，N）的日变化（D）而变化，拟合度很好，符合改进的逻辑斯谛方程：I=0.91/[1+exp(8.5+(2.0 HT HRH-20.2 NI0)D)]（r(2)=0.897），其中 HT 表示日低温时间（<4°C），HRH 表示日高气湿时间（>90%RH），I0 表示初始感染水平。该模型表明了非生物和生物因素对冬季球孢白僵菌真菌病发展的影响，也验证了真菌通过感染可用的宿主蚜虫而无需休眠阶段来越冬。最终，球孢白僵菌真菌病使蚜虫种群减少了 81.4%，具有很大的生物防治潜力。
