Laboratoire de Biotechnologie Microbienne, Faculté des Sciences et Techniques de Fès , B.P: 2202, Fès , Maroc.
Braz J Microbiol. 2012 Oct;43(4):1516-22. doi: 10.1590/S1517-838220120004000036. Epub 2012 Jun 1.
The treatment of tuberculosis has become more difficult with the worldwide spread of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of Mycobacterium tuberculosis. Moreover, the prevalence of human disease caused by atypical mycobacteria has also increased in the past two decades and has further complicated the problem of the treatment of mycobacterial infections. It is therefore urgent to develop new highly active molecules against these bacteria. The present study reports the isolation from a Moroccan soil of a Bacillus strain that exhibits an important antimycobacterial activity. The strain was identified as Brevibacillus laterosporus using DNA sequencing of the 16S ribosomal RNA gene. The antimycobacterial activity was assigned to a substance with a protein nature. This nature was revealed using a liquid-liquid extraction with organic solvents, precipitation with ammonium sulfate and treatment with a protease. This study suggested the identification and the characterization of this active metabolite enabling therapeutic investigations further.
随着全球耐多药（MDR）和广泛耐药（XDR）结核分枝杆菌菌株的传播，结核病的治疗变得更加困难。此外，过去二十年中，由非典型分枝杆菌引起的人类疾病的患病率也有所增加，这进一步使分枝杆菌感染的治疗问题复杂化。因此，迫切需要开发针对这些细菌的新型高活性分子。本研究报告了从摩洛哥土壤中分离出一株具有重要抗分枝杆菌活性的芽孢杆菌。该菌株通过 16S 核糖体 RNA 基因的 DNA 测序被鉴定为短芽孢杆菌。抗分枝杆菌活性被分配给一种具有蛋白质性质的物质。使用有机溶剂的液-液萃取、硫酸铵沉淀和蛋白酶处理来揭示这种性质。本研究建议鉴定和表征这种活性代谢物，以便进一步进行治疗研究。
