Wang Jing, Chen Xuewei, Zhang Na, Ma Qiang
Department of Occupational Hygiene, Tianjin Institute of Health and Environmental Medicine Tianjin, People's Republic of China.
Chin J Physiol. 2013 Oct 31;56(5):245-52. doi: 10.4077/CJP.2013.BAB097. Epub 2013 Aug 31.
Exercise is beneficial to brain and can attenuate stress-induced hippocampal damages. However, the details involved monoamine neurotransmitter in exercise to counteract stress have not been well elucidated. The aim of this study was to examine exercise-induced responses of the norepinephrine (NE) and serotonin (5-HT) systems in counteracting stress-induced hippocampal damages. Rats were divided into exercise (four weeks of voluntary wheel running), stress (three weeks of restraint stress), exercise-stress (three weeks of stress following four weeks of exercise), and control groups. Levels of NE and 5-HT were detected with high-performance liquid chromatography (HPLC), mRNA expression was detected with real-time fluorescence quantitative reverse transcription polymerase reaction (FQ-RT-PCR) and proteins associated with 5-HT₁Α receptors (5-HT₁Α-R) and β₂-adrenergic receptors (β₂-AR) were analyzed by western blotting. 5-HT levels were highest (P < 0.01) in the exercised group, lowest (P < 0.05) in the stressed rats, and were similar (P = 0.065) in stressed and exercise-stressed rats. NE levels were highest (P < 0.01) in the exercised group, and higher in the exercise-stressed than the stressed rats (P < 0.01). 5-HT₁A-R mRNA expression was highest (P < 0.01) in the exercised group, lowest in the stressed group. The 5-HT₁Α-R protein expression changed in the same tendency as its mRNA levels. The β₂-AR mRNA was highest in exercised rats (P < 0.05), and its protein expression was higher in the exercised and exercise-stress rats than in the control and stress rats (P < 0.05). In conclusion, norepinephrine may represent endophenotypic features of exercise states. Serotonin levels may be more susceptible to stress and responsible for deleterious stress-induced effects. Norepinephrine and serotonin may both contribute to counteraction of stress-induced hippocampal damages of physical exercises.
运动有益于大脑，并且可以减轻应激诱导的海马体损伤。然而，运动过程中涉及单胺神经递质以对抗应激的具体细节尚未得到充分阐明。本研究的目的是检测运动诱导的去甲肾上腺素（NE）和5-羟色胺（5-HT）系统在对抗应激诱导的海马体损伤中的反应。将大鼠分为运动组（四周自愿轮转跑步）、应激组（三周束缚应激）、运动-应激组（四周运动后三周应激）和对照组。采用高效液相色谱法（HPLC）检测NE和5-HT水平，采用实时荧光定量逆转录聚合酶反应（FQ-RT-PCR）检测mRNA表达，并通过蛋白质印迹法分析与5-HT₁Α受体（5-HT₁Α-R）和β₂-肾上腺素能受体（β₂-AR）相关的蛋白质。运动组的5-HT水平最高（P < 0.01），应激大鼠中最低（P < 0.05），应激组和运动-应激组相似（P = 0.065）。运动组的NE水平最高（P < 0.01），运动-应激组高于应激大鼠（P < 0.01）。运动组的5-HT₁A-R mRNA表达最高（P < 0.01），应激组最低。5-HT₁Α-R蛋白表达与其mRNA水平变化趋势相同。β₂-AR mRNA在运动大鼠中最高（P < 0.05），其蛋白表达在运动组和运动-应激组大鼠中高于对照组和应激组大鼠（P < 0.05）。总之，去甲肾上腺素可能代表运动状态的内表型特征。5-羟色胺水平可能对应激更敏感，并导致有害的应激诱导效应。去甲肾上腺素和5-羟色胺可能都有助于对抗体育锻炼应激诱导的海马体损伤。
