Konturek S J, Bielański W, Solomon T E
Institute of Physiology, Medical Academy, Krakow, Poland.
Gastroenterology. 1990 Jan;98(1):47-55. doi: 10.1016/0016-5085(90)91289-i.
The purpose of this study was to determine the role of gastrin and cholecystokinin in the cephalic phase of gastrin release and gastric and pancreatic secretion in conscious dogs. Sham feeding in intact dogs increased gastric acid output to about 65% of histamine maximum and pancreatic protein to 23% of caerulein maximum. Significant increases in plasma gastrin and pancreatic polypeptide but not cholecystokinin occurred. Similar effects were obtained using insulin hypoglycemia or 2-deoxy-D-glucose glucocytopemia. Atropine eliminated gastric acid response to sham feeding, insulin, or 2-deoxy-D-glucose, significantly reduced the pancreatic protein response by about 60%, and abolished plasma pancreatic polypeptide but not plasma gastrin. Blocking of cholecystokinin receptors by L-364,715 did not affect gastric or pancreatic secretory responses to sham feeding, insulin, or 2-deoxy-D-glucose and failed to influence the accompanying increments in plasma gastrin and pancreatic polypeptide. In antral-mucosectomized dogs, sham feeding-induced acid output reached only 17% of histamine maximum but the increase in pancreatic protein output was similar to that in intact dogs. In these animals, background stimulation with G17I (62 pmol/kg per h) potentiated the gastric acid response to sham feeding but had little effect on pancreatic protein output. This study provides evidence that unlike gastric acid, the pancreatic protein response to physiological or pharmacological cephalic stimulation does not depend on vagally released gastrin but probably on direct vagal stimulation of the pancreas.
本研究的目的是确定胃泌素和胆囊收缩素在清醒犬胃泌素释放、胃和胰腺分泌的头期阶段所起的作用。对完整的犬进行假饲，可使胃酸分泌量增加至组胺最大分泌量的约65%，胰腺蛋白分泌量增加至蛙皮素最大分泌量的23%。血浆胃泌素和胰腺多肽显著增加，但胆囊收缩素未增加。使用胰岛素低血糖或2-脱氧-D-葡萄糖引起的糖血症也获得了类似的效果。阿托品消除了对假饲、胰岛素或2-脱氧-D-葡萄糖的胃酸反应，使胰腺蛋白反应显著降低约60%，并消除了血浆胰腺多肽，但未消除血浆胃泌素。L-364,715阻断胆囊收缩素受体并不影响对假饲、胰岛素或2-脱氧-D-葡萄糖的胃或胰腺分泌反应，也未能影响血浆胃泌素和胰腺多肽的伴随增加。在切除胃窦黏膜的犬中，假饲诱导的胃酸分泌量仅达到组胺最大分泌量的17%，但胰腺蛋白分泌量的增加与完整犬相似。在这些动物中，用G17I（62 pmol/kg每小时）进行背景刺激可增强对假饲的胃酸反应，但对胰腺蛋白分泌量影响不大。本研究提供的证据表明，与胃酸不同，胰腺蛋白对生理或药理头期刺激的反应不依赖于迷走神经释放的胃泌素，而可能依赖于迷走神经对胰腺的直接刺激。
