逆转录聚合酶链反应（RT-PCR）。
Reverse-transcription PCR (RT-PCR).
作者信息
Bachman Julia
机构信息
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
出版信息
Methods Enzymol. 2013;530:67-74. doi: 10.1016/B978-0-12-420037-1.00002-6.
RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g., see Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates and Circularized RT-PCR (cRT-PCR): analysis of RNA 5' ends, 3' ends, and poly(A) tails).
逆转录聚合酶链反应（RT-PCR）通常用于检测遗传疾病，以及在各种组织类型、细胞类型和发育时间进程中表征基因表达。这是一种表达谱分析形式，但通常作为一种候选方法。RT-PCR也常用于克隆互补DNA（cDNA），以便与其他分子生物学技术进一步联用（例如，见寡聚（dT）引物逆转录聚合酶链反应分离聚腺苷酸化RNA降解中间体和环化逆转录聚合酶链反应（cRT-PCR）：RNA 5'端、3'端和聚（A）尾的分析）。
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