用于定量实时PCR的（沃克）内参基因的选择
Selection of (Walker) Reference Genes for Quantitative Real-Time PCR.
作者信息
Zhai Yutong, Yu Yonghao, Xu Pengfei, Zeng Xianru, Long Xiuzhen, Wei Dewei, He Zhan, Gao Xuyuan
机构信息
Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Nanning 530007, China.
出版信息
Insects. 2025 Apr 24;16(5):445. doi: 10.3390/insects16050445.
The selection of appropriate reference genes is critical for standardizing quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) data, thereby ensuring accurate and reliable results of gene expression analysis. In this study, we identified 10 candidate reference genes (encoding , , , , , , , , , and ) and evaluated their suitability for use as reference genes in the pest insect, . Analysis was conducted using three software-based methods-Delta CT, NormFinder, and BestKeeper-alongside the online tool RefFinder. Expression levels of these genes were analyzed across various developmental stages and body parts. The overall ranking of reference gene stability scores was as follows: > > > > > > > > > . Ultimately, and were identified as the most stable reference genes for .
选择合适的内参基因对于标准化定量实时逆转录聚合酶链反应（RT-qPCR）数据至关重要，从而确保基因表达分析结果的准确可靠。在本研究中，我们鉴定了10个候选内参基因（分别编码 、 、 、 、 、 、 、 、 和 ），并评估了它们作为害虫 内参基因的适用性。使用基于软件的三种方法——Delta CT、NormFinder和BestKeeper，以及在线工具RefFinder进行分析。在不同发育阶段和身体部位分析了这些基因的表达水平。内参基因稳定性评分的总体排名如下： > > > > > > > > > 。最终， 和 被确定为 最稳定的内参基因。
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