Temsamani Fadoua, Agalit Assia, Idrissi Serhrouchni Karima
Laboratory of Histology, Embryology and Cytogenetics, Laboratory of Life and Health Sciences Research, Faculty of Medicine and Pharmacy of Tangier, Abdelmalek Essaâdi University, Tangier, MAR.
Cureus. 2025 Jan 13;17(1):e77362. doi: 10.7759/cureus.77362. eCollection 2025 Jan.
Pluripotent stem cells (PSCs) have revolutionized medical research by offering significant advancements in regenerative medicine, disease modeling, and drug discovery. Several research and clinical studies have successfully generated PSCs but with varying characterization methodologies. Characterization methods are crucial to guarantee the safety and efficacy of PSCs in research and therapeutic applications. Therefore, there is a need for standardization of characterization methods to ensure their quality, reproducibility, and comparability across laboratories. In this review, the authors discuss the mandatory and non-mandatory assessments used for morphological, molecular, genetic, and functional PSC characterization. We will present their advantages and limits with the aim of providing insights for assessment optimization and helping facilitate the standardization of characterization methods.
多能干细胞（PSCs）通过在再生医学、疾病建模和药物发现等方面取得重大进展，彻底改变了医学研究。多项研究和临床研究已成功生成PSCs，但所采用的表征方法各不相同。表征方法对于确保PSCs在研究和治疗应用中的安全性和有效性至关重要。因此，需要对表征方法进行标准化，以确保其质量、可重复性以及不同实验室之间的可比性。在本综述中，作者讨论了用于PSCs形态学、分子、遗传和功能表征的强制性和非强制性评估。我们将阐述它们的优点和局限性，旨在为评估优化提供见解，并有助于促进表征方法的标准化。
