Rodríguez Javier O, Prieto Signed E, Correa Catalina, Pérez Carlos E, Mora Jessica T, Bravo Juan, Soracipa Yolanda, Alvarez Luisa F
Insight Group, Research Area and Special Internship "Mathematical and Physical Theories Applied to Medicine", Medicine Faculty, Universidad Militar Nueva Granada, Cr 11 No,101-80 Bogotá, Colombia.
BMC Med Phys. 2013 Sep 14;13(1):3. doi: 10.1186/1756-6649-13-3.
HIV diagnosis, prognostic and treatment requires T CD4 lymphocytes' number from flow cytometry, an expensive technique often not available to people in developing countries. The aim of this work is to apply a previous developed methodology that predicts T CD4 lymphocytes' value based on total white blood cell (WBC) count and lymphocytes count applying sets theory, from information taken from the Complete Blood Count (CBC).
Sets theory was used to classify into groups named A, B, C and D the number of leucocytes/mm3, lymphocytes/mm3, and CD4/μL3 subpopulation per flow cytometry of 800 HIV diagnosed patients. Union between sets A and C, and B and D were assessed, and intersection between both unions was described in order to establish the belonging percentage to these sets. Results were classified into eight ranges taken by 1000 leucocytes/mm3, calculating the belonging percentage of each range with respect to the whole sample.
Intersection (A ∪ C) ∩ (B ∪ D) showed an effectiveness in the prediction of 81.44% for the range between 4000 and 4999 leukocytes, 91.89% for the range between 3000 and 3999, and 100% for the range below 3000.
Usefulness and clinical applicability of a methodology based on sets theory were confirmed to predict the T CD4 lymphocytes' value, beginning with WBC and lymphocytes' count from CBC. This methodology is new, objective, and has lower costs than the flow cytometry which is currently considered as Gold Standard.
HIV的诊断、预后评估及治疗需要通过流式细胞术检测T CD4淋巴细胞数量，而这项技术成本高昂，发展中国家的人们往往难以获得。本研究的目的是应用一种先前开发的方法，该方法基于全血细胞计数（CBC）中的信息，运用集合论，根据白细胞总数（WBC）和淋巴细胞计数来预测T CD4淋巴细胞的值。
运用集合论将800例HIV确诊患者的流式细胞术检测的每立方毫米白细胞数、每立方毫米淋巴细胞数及每微升CD4细胞亚群数量分为A、B、C和D组。评估集合A与C、B与D的并集，并描述两个并集的交集，以确定各集合的归属百分比。结果分为以1000个白细胞每立方毫米为间隔的八个范围，计算每个范围相对于整个样本的归属百分比。
交集（A∪C）∩（B∪D）在预测白细胞数在4000至4999之间的范围时有效率为81.44%，在3000至3999之间的范围时有效率为91.89%，在低于3000的范围时有效率为100%。
基于集合论的方法从CBC中的WBC和淋巴细胞计数开始预测T CD4淋巴细胞值的实用性和临床适用性得到了证实。该方法新颖、客观，且成本低于目前被视为金标准的流式细胞术。
