Department of Biotechnology, National Institute of Technology Durgapur, Mahatma Gandhi Avenue, Durgapur 713209, India.
Department of Bio Engineering, National Institute of Technology Agartala, Barjala, Tripura 799055, India.
Bioresour Technol. 2013 Nov;147:662-666. doi: 10.1016/j.biortech.2013.08.117. Epub 2013 Aug 28.
A novel white rot fungus Alternaria alternata CMERI F6 decolorized 99.99% of 600 mg/L congo red within 48 h in yeast extract-glucose medium at 25 °C, pH 5 and 150 rpm. Physicochemical parameters like carbon and nitrogen sources, temperature, pH and aeration were optimized to develop faster decolorization process. Dye decolorization rate was maximal (20.21 mg/L h) at 25 °C, pH 5, 150 rpm and 800 mg/L dye, giving 78% final decolorization efficiency. Scanning electron microscopy and X-ray Diffraction analysis revealed that the fungus become amorphous after dye adsorption. HPLC and FTIR analysis of the extracted metabolites suggested that the decolorization occurred through biosorption and biodegradation. Thermogravimetric analysis (TGA), differential thermal analysis (DTA) and acid-alkali and 70% ethanol treatment revealed the efficient dye retention capability of the fungus. The foregoing results justify the applicability of the strain in removal of congo red from textile wastewaters and their safe disposal.
一种新型白腐真菌交替单端孢霉 CMERI F6 在 25°C、pH5 和 150rpm 的酵母葡萄糖培养基中，48 小时内可将 600mg/L 的刚果红脱色 99.99%。为了开发更快的脱色过程，对碳氮源、温度、pH 值和通气等理化参数进行了优化。在 25°C、pH5、150rpm 和 800mg/L 染料条件下，染料脱色率最高（20.21mg/L h），最终脱色效率达到 78%。扫描电子显微镜和 X 射线衍射分析表明，真菌在吸附染料后变得无定形。提取代谢物的高效液相色谱和傅里叶变换红外分析表明，脱色是通过生物吸附和生物降解发生的。热重分析（TGA）、差热分析（DTA）、酸碱和 70%乙醇处理表明，真菌对染料具有高效的保留能力。上述结果证明了该菌株在去除纺织废水中刚果红及其安全处理方面的适用性。
