Enzyme Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Khlong Luang, Pathumthani, 12120, Thailand.
Food Biotechnology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Khlong Luang, Pathumthani, 12120, Thailand.
World J Microbiol Biotechnol. 2023 Jun 15;39(9):226. doi: 10.1007/s11274-023-03672-7.
Wastewater from the textile industry dyeing process containing high loads of synthetic dyes leads to pollution of water with these toxic and genotoxic dyes. Much effort has been put towards developing biological systems to resolve this issue. Mycoremediation is a well-known approach using fungi to remove, degrade, or remediate pollutants and can be applied to decolorize textile dyes in industrial effluent. Fungal strains from four genera of Polyporales, namely Coriolopsis sp. TBRC 2756, Fomitopsis pinicola TBRC-BCC 30881, Rigidoporus vinctus TBRC 6770, and Trametes pocas TBRC-BCC 18705, were studied for decolorization efficiency, and R. vinctus was found to exhibit the greatest activity in removing all seven tested reactive dyes and one acid dye with a decolorization efficiency of 80% or more within 7 days under limited oxygen. This fungus simultaneously degraded multiple dyes in synthetic wastewater as well as industrial effluent from the dyeing process. To enhance the decolorization rate, various fungal consortia were formulated for testing. However, these consortia only trivially improved efficiency compared with using R. vinctus TBRC 6770 alone. Evaluation of R. vinctus TBRC 6770 decolorization ability was further performed in a 15-L bioreactor to test its ability to eliminate multiple dyes from industrial effluent. The fungus took 45 days to adapt to growth in the bioreactor and subsequently reduced dye concentration to less than 10% of the initial concentration. The following six cycles required only 4-7 days to reduce dye concentrations to less than 25%, demonstrating that the system can run efficiently for multiple cycles without the need for extra medium or other carbon sources.
纺织工业染色过程产生的废水含有高浓度的合成染料，导致这些有毒和遗传毒性染料对水造成污染。人们已经投入大量精力开发生物系统来解决这个问题。真菌修复是一种利用真菌去除、降解或修复污染物的知名方法，可用于在工业废水中对纺织染料进行脱色。研究了来自四个多孔菌纲属的真菌菌株，即卷边革孔菌 TBRC 2756、胶膜革孔菌 TBRC-BCC 30881、纤孔菌 TBRC 6770 和粗毛栓菌 TBRC-BCC 18705，以研究其脱色效率，结果发现纤孔菌 TBRC 6770 在有限氧条件下 7 天内对 7 种测试的活性染料和 1 种酸性染料的脱色效率达到 80%或更高，显示出最大的活性。该真菌同时在合成废水中以及在染色过程的工业废水中降解多种染料。为了提高脱色率，对各种真菌共生体进行了配方测试。然而，与单独使用纤孔菌 TBRC 6770 相比，这些共生体仅略微提高了效率。进一步在 15-L 生物反应器中评估纤孔菌 TBRC 6770 的脱色能力，以测试其从工业废水中去除多种染料的能力。该真菌需要 45 天才能适应生物反应器中的生长，随后将染料浓度降低到初始浓度的 10%以下。随后的六个周期仅需要 4-7 天即可将染料浓度降低到 25%以下，表明该系统可以在无需额外培养基或其他碳源的情况下高效运行多个周期。
