Department of Pathology, Affiliated Zhongda Hospital Southeast University, 87 Dingjiaqiao, Nanjing, Jiangsu 210009, China.
Pathol Res Pract. 2013 Oct;209(10):611-7. doi: 10.1016/j.prp.2013.03.012. Epub 2013 Apr 28.
SIRT1 and p300/CBP, which are considered to be essential histone deacetylases and acetyltransferases, are also considered to be relative to tumorigenesis because they modulate the expression of several tumor suppressor genes. Therefore, this study investigated the expression of SIRT1 and p300/CBP in gastroesophageal junction (GEJ) cancer and their correlation with E-cadherin and MLH1 in order to explore the clinicopathological significance of SIRT1 and p300/CBP expression and their possible effects involving E-cadherin and MLH1 expression. Tissue microarray technique and immunohistochemical stains were applied to evaluate the SIRT1, p300/CBP, E-cadherin, and MLH1 expression in 176 GEJ cancer tissues and 32 normal GEJ region tissues. The results showed that the over-expression of SIRT1 was associated with a higher number of metastasis lymph nodes, more advanced staging, and shorter mean survival time. SIRT1 and p300/CBP were negatively and positively correlated with the expression of E-cadherin and MLH1, respectively, in the cancer cases. These results indicated a possible effect of SIRT1 and p300/CBP involved in regulating the expression of E-cadherin and MLH1, thus participating in the tumor progression of GEJ cancer.
SIRT1 和 p300/CBP 被认为是重要的组蛋白去乙酰化酶和乙酰转移酶，也被认为与肿瘤发生有关，因为它们调节多个肿瘤抑制基因的表达。因此，本研究调查了 SIRT1 和 p300/CBP 在胃食管交界处 (GEJ) 癌中的表达及其与 E-钙黏蛋白和 MLH1 的相关性，以探讨 SIRT1 和 p300/CBP 表达的临床病理意义及其对 E-钙黏蛋白和 MLH1 表达的可能影响。应用组织微阵列技术和免疫组织化学染色法评估了 176 例 GEJ 癌组织和 32 例正常 GEJ 区组织中的 SIRT1、p300/CBP、E-钙黏蛋白和 MLH1 的表达。结果表明，SIRT1 的过度表达与更多的转移淋巴结、更晚期的分期和更短的平均生存时间有关。在癌症病例中，SIRT1 和 p300/CBP 分别与 E-钙黏蛋白和 MLH1 的表达呈负相关和正相关。这些结果表明 SIRT1 和 p300/CBP 可能通过调节 E-钙黏蛋白和 MLH1 的表达来参与 GEJ 癌的肿瘤进展。
