重组人粒细胞巨噬细胞集落刺激因子的结晶及初步X射线研究
Crystallization and preliminary x-ray investigation of recombinant human granulocyte-macrophage colony-stimulating factor.
作者信息
Reichert P, Cook W J, Ealick S E, Hammond G S, Le H V, Nagabhushan T L, Trotta P P, Bugg C E
机构信息
Schering Corporation, Bloomfield, New Jersey 07003.
出版信息
J Biol Chem. 1990 Jan 5;265(1):452-3.
Crystals of recombinant human granulocyte-macrophage colony-stimulating factor have been grown from solutions of polyethylene glycol 8000. The crystals are orthorhombic, space group P2(1)2(1)2(1); the axes are a = 45.5(1), b = 58.7(1) and c = 127.3(1) A. The crystals are stable to x-rays for at least 3 days and diffract beyond 2.8-A resolution. Although the molecule exists as a monomer in solution, it crystallizes with two or three molecules in the asymmetric unit.
重组人粒细胞巨噬细胞集落刺激因子晶体已从聚乙二醇8000溶液中生长出来。晶体为正交晶系，空间群P2(1)2(1)2(1)；轴长分别为a = 45.5(1)、b = 58.7(1)和c = 127.3(1)埃。这些晶体对X射线至少稳定3天，且能衍射到2.8埃以上的分辨率。尽管该分子在溶液中以单体形式存在，但在不对称单元中结晶时含有两个或三个分子。
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