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重组莱氏乳杆菌核苷脱氧核糖基转移酶的结晶及初步X射线研究

Crystallization and preliminary X-ray investigation of recombinant Lactobacillus leichmannii nucleoside deoxyribosyltransferase.

作者信息

Cook W J, Short S A, Ealick S E

机构信息

Department of Pathology, University of Alabama, Birmingham 35294.

出版信息

J Biol Chem. 1990 Feb 15;265(5):2682-3.

PMID:2406239
Abstract

Crystals of recombinant bacterial nucleoside deoxyribosyltransferase have been grown from solutions of ammonium sulfate. The crystals are cubic, space group I23 or I2(1)3; the axial length is 151.1(2) A. The crystals are stable to x-rays for at least 5 days and diffract beyond 2.8-A resolution. It appears that the molecule, which is a hexamer, utilizes the symmetry of the space group, resulting in two or three subunits per asymmetric unit.

摘要

重组细菌核苷脱氧核糖基转移酶的晶体已从硫酸铵溶液中生长出来。这些晶体呈立方体,空间群为I23或I2(1)3;轴长为151.1(2)埃。这些晶体对X射线至少稳定5天,并且能衍射到2.8埃分辨率以上。看来该分子是一个六聚体,利用了空间群的对称性,导致每个不对称单元中有两个或三个亚基。

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