Haaf T, Groscurth A G, Machens A, Schmid M
Department of Human Genetics, University of Würzburg, Federal Republic of Germany.
J Histochem Cytochem. 1990 Jan;38(1):129-34. doi: 10.1177/38.1.2403577.
Immunofluorescence staining using human autoantibodies is a simple and reliable method for investigation of meiotic and post-meiotic cells. Patients suffering from autoimmune diseases often produce circulating autoantibodies to antigens of germ cells and Sertoli cells. Four hundred human autoimmune sera were screened by indirect immunofluorescence on mouse seminiferous tubule cells. Autoantibodies of several specificities were found: one group reacted with organelles of meiotic prophase spermatocytes or spermatozoa. Included in this group were autoantibodies to synaptonemal complexes, sex vesicle, acrosome, and sperm tail. A second group of autoantibodies was found to stain different spermatogenic cell types uniformly, such as round spermatids or Sertoli cells.
使用人类自身抗体进行免疫荧光染色是研究减数分裂和减数分裂后细胞的一种简单可靠的方法。患有自身免疫性疾病的患者通常会产生针对生殖细胞和支持细胞抗原的循环自身抗体。通过对小鼠曲细精管细胞进行间接免疫荧光筛选了400份人类自身免疫血清。发现了几种特异性的自身抗体：一组与减数分裂前期精母细胞或精子的细胞器发生反应。该组包括针对联会复合体、性泡、顶体和精子尾部的自身抗体。发现第二组自身抗体能均匀地对不同类型的生精细胞进行染色，如圆形精子细胞或支持细胞。
