Alqasoumi Saleh Ibrahim, Farraj Abdallah Ibrahim, Abdel-Kader Maged Saad
Department of Pharmacognosy, College of Pharmacy,King Saud University, Riyadh, Kingdom of Saudi Arabia.
Pak J Pharm Sci. 2013 Sep;26(5):999-1008.
Different fraction obtained from the aerial parts of Juniperus phoenicea showed significant activity as hepatoprotective when investigated against carbon tetrachloride induced liver injury. The hepatoprotective activity was evaluated through the quantification of biochemical parameters and confirmed using histopathology study. Phytochemical investigation of the petroleum ether, chloroform and methanol fractions utilizing different chromatographic techniques resulted in the isolation of five known diterpenoids namely: 13-epicupressic acid (1), imbricatolic acid (2), 7α-hydroxysandaracopimaric acid (3), 3β-hydroxysandaracopimaric acid (4), isopimaric acid (5), four flavonoid derivatives: cupressuflavone (6), hinokiflavone (7), hypolaetin-7-O-β-xylopyranoside (9), (-) catechin (10), inaddition to sucrose (8). Both physical and spectral data were used for structure determination and all isolates were evaluated for their hepatoprotective activity. Compounds 2 and 6 were effective, however; 7 was the most active. Hepatoprotective activity of 7 is comparable with the standard drug silymarin in reducing the elevated liver enzymes and restoring normal appearance of hepatocytes. Hepatoprotective effect of combination of 6, 7 and silymarin with the diterpene sugiol was also explored.
从刺柏地上部分获得的不同馏分，在针对四氯化碳诱导的肝损伤进行研究时，显示出显著的保肝活性。通过对生化参数进行定量来评估保肝活性，并使用组织病理学研究加以证实。利用不同色谱技术对石油醚、氯仿和甲醇馏分进行植物化学研究，分离出了5种已知的二萜类化合物，即：13-表柏木酸（1）、叠生酸（2）、7α-羟基山达海松酸（3）、3β-羟基山达海松酸（4）、异海松酸（5），4种黄酮类衍生物：柏黄酮（6）、扁柏黄酮（7）、次甲基鼠李素-7-O-β-木糖苷（9）、（-）儿茶素（10），此外还有蔗糖（8）。利用物理和光谱数据确定结构，并对所有分离物的保肝活性进行评估。化合物2和6有效果，然而；7的活性最强。7的保肝活性在降低升高的肝酶和恢复肝细胞正常外观方面与标准药物水飞蓟素相当。还探索了6、7和水飞蓟素与二萜类化合物苏乔醇联合使用的保肝作用。
