Abdel-Kader Maged S, Soliman Gamal A, Alqarni Mohammed H, Hamad Abubaker M, Foudah Ahmed I, Alqasoumi Saleh I
Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia.
Department of Pharmacognosy, College of Pharmacy, Alexandria University, Alexandria 21215, Egypt.
Saudi Pharm J. 2019 Nov;27(7):945-951. doi: 10.1016/j.jsps.2019.07.003. Epub 2019 Jul 15.
The hepatoprotective activity of the total extract of L. against CCl induced toxicity in experimental animals was previously reported and indicated promising results. Essential oil of was prepared by hydrodistillation method. Components of the oil were identified by comparison of GC-MS and retention indexes with reported data. The hepatoprotective effect of the essential oil against CCl induced toxicity was studied using male Wistar rats and silymarin at 10 mg/kg p.o as standard drug. The protective effect was evaluated via serum biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyltranspeptidase (GGT), and total bilirubin as well as tissue parameters including non-protein sulfhydryl groups (NP-SH), malonaldehyde (MDA) and total protein (TP). Histopathological study was applied on the liver tissues using Mayer's hematoxylin stain, Periodic Acid Schiff - Hematoxylin (PAS-H) and Masson trichrome technique on light microscope. Electron microscope images were also obtained for more detailed study.
先前已有报道称，L. 的总提取物对实验动物中四氯化碳诱导的毒性具有肝保护活性，并显示出有前景的结果。通过水蒸馏法制备了 的精油。通过气相色谱 - 质谱联用（GC-MS）以及保留指数与已报道数据的比较来鉴定该油的成分。以10毫克/千克口服的水飞蓟宾作为标准药物，使用雄性Wistar大鼠研究了该精油对四氯化碳诱导毒性的肝保护作用。通过血清生化参数如天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、γ-谷氨酰转肽酶（GGT）和总胆红素，以及组织参数包括非蛋白巯基（NP-SH）、丙二醛（MDA）和总蛋白（TP）来评估保护作用。使用梅耶苏木精染色、高碘酸希夫 - 苏木精（PAS-H）和马松三色染色技术在光学显微镜下对肝组织进行组织病理学研究。还获得了电子显微镜图像以进行更详细的研究。
