Key Laboratory of Molecular Biology on Infection Diseases of the Ministry of Education, Chongqing Medical University, Chongqing 400016, China; Department of Microbiology and Immunology, North Sichuan Medical College, Nanchong 637000, China.
J Virol Methods. 2013 Dec;194(1-2):277-9. doi: 10.1016/j.jviromet.2013.08.034. Epub 2013 Sep 13.
The relax circle DNA (rcDNA) sequence and the covalently closed circle DNA (cccDNA) sequence in hepatitis B virus (HBV) are crucial regions for HBV infections. To analyze mutations in rcDNA and cccDNA, DNA sequencing is often used, although it is time-consuming and expensive. Herein, we report a simple, economic, albeit accurate allele-specific polymerase chain reaction (AS-PCR) to detect mutations in these regions of HBV. This method can be extensively used to screen for mutations at specific positions of HBV genome.
乙型肝炎病毒 (HBV) 的松弛环状 DNA (rcDNA) 序列和共价闭合环状 DNA (cccDNA) 序列是 HBV 感染的关键区域。为了分析 rcDNA 和 cccDNA 中的突变，通常使用 DNA 测序，但这种方法既耗时又昂贵。在此，我们报告了一种简单、经济但准确的等位基因特异性聚合酶链反应 (AS-PCR) 方法，用于检测 HBV 这些区域的突变。该方法可广泛用于筛选 HBV 基因组特定位置的突变。
