Wahadoszamen Md, Ghazaryan Artur, Cingil Hande E, Ara Anjue Mane, Büchel Claudia, van Grondelle Rienk, Berera Rudi
Division of Physics and Astronomy, Department of Biophysics, VU University Amsterdam, The Netherlands; Department of Physics, University of Dhaka, Dhaka 1000, Bangladesh.
Biochim Biophys Acta. 2014 Jan;1837(1):193-200. doi: 10.1016/j.bbabio.2013.09.001. Epub 2013 Sep 11.
Diatoms are characterized by very efficient photoprotective mechanisms where the excess energy is dissipated as heat in the main antenna system constituted by fucoxanthin-chlorophyll (Chl) protein complexes (FCPs). We performed Stark fluorescence spectroscopy on FCPs in their light-harvesting and energy dissipating states. Our results show that two distinct emitting bands are created upon induction of energy dissipation in FCPa and possibly in FCPb. More specifically one band is characterized by broad red shifted emission above 700nm and bears strong similarity with a red shifted band that we detected in the dissipative state of the major light-harvesting complex II (LHCII) of plants [26]. We discuss the results in the light of different mechanisms proposed to be responsible for photosynthetic photoprotection.
硅藻具有非常高效的光保护机制，其中多余的能量在由岩藻黄素 - 叶绿素（Chl）蛋白复合物（FCPs）构成的主要天线系统中以热的形式耗散。我们对处于光捕获和能量耗散状态的FCPs进行了斯塔克荧光光谱分析。我们的结果表明，在FCPa以及可能在FCPb中诱导能量耗散时会产生两个不同的发射带。更具体地说，一个带的特征是在700nm以上有宽的红移发射，并且与我们在植物主要光捕获复合物II（LHCII）的耗散状态中检测到的红移带非常相似。我们根据提出的负责光合光保护的不同机制来讨论这些结果。
