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使用消费级扫描仪的在线数字全息显微镜。

In-line digital holographic microscopy using a consumer scanner.

作者信息

Shimobaba Tomoyoshi, Yamanashi Hiroya, Kakue Takashi, Oikawa Minoru, Okada Naohisa, Endo Yutaka, Hirayama Ryuji, Masuda Nobuyuki, Ito Tomoyoshi

机构信息

Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inege-ku, Chiba 263-8522, Japan.

出版信息

Sci Rep. 2013;3:2664. doi: 10.1038/srep02664.

DOI:10.1038/srep02664
PMID:24036588
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3773619/
Abstract

We demonstrate an in-line digital holographic microscopy using a consumer scanner. The consumer scanner can scan an image with 4,800 dpi. The pixel pitch is approximately 5.29 μm. The system using a consumer scanner has a simple structure, compared with synthetic aperture digital holography using a camera mounted on a two-dimensional moving stage. In this demonstration, we captured an in-line hologram with 23, 602 × 18, 023 pixels (≈0.43 gigapixels). The physical size of the scanned hologram is approximately 124 mm × 95 mm. In addition, to accelerate the reconstruction time of the gigapixel hologram and decrease the amount of memory for the reconstruction, we applied the band-limited double-step Fresnel diffraction to the reconstruction.

摘要

我们展示了一种使用消费级扫描仪的同轴数字全息显微镜。该消费级扫描仪能够以4800 dpi扫描图像。像素间距约为5.29μm。与使用安装在二维移动平台上的相机的合成孔径数字全息术相比,使用消费级扫描仪的系统结构简单。在本次演示中,我们捕获了一幅具有23602×18023像素(≈0.43千兆像素)的同轴全息图。扫描全息图的物理尺寸约为124 mm×95 mm。此外,为了加快千兆像素全息图的重建时间并减少重建所需的内存量,我们在重建中应用了带限双步菲涅耳衍射。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/3773619/d54d3508141e/srep02664-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/3773619/b56d8be25f05/srep02664-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/3773619/c3793e10a760/srep02664-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/3773619/ce23cab28c9a/srep02664-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/3773619/7fa76373f29e/srep02664-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/3773619/d54d3508141e/srep02664-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/3773619/b56d8be25f05/srep02664-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/3773619/c3793e10a760/srep02664-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/3773619/ce23cab28c9a/srep02664-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/3773619/7fa76373f29e/srep02664-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a78e/3773619/d54d3508141e/srep02664-f5.jpg

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本文引用的文献

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Band-limited double-step Fresnel diffraction and its application to computer-generated holograms.带限双步菲涅耳衍射及其在计算全息图中的应用。
Opt Express. 2013 Apr 8;21(7):9192-7. doi: 10.1364/OE.21.009192.
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Giga-pixel lensfree holographic microscopy and tomography using color image sensors.使用彩色图像传感器的吉兆像素无透镜全息显微镜和层析成像。
PLoS One. 2012;7(9):e45044. doi: 10.1371/journal.pone.0045044. Epub 2012 Sep 12.
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Sensors (Basel). 2019 Mar 11;19(5):1234. doi: 10.3390/s19051234.
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High resolution digital holographic microscopy with a wide field of view based on a synthetic aperture technique and use of linear CCD scanning.基于合成孔径技术和线性电荷耦合器件扫描的具有宽视场的高分辨率数字全息显微镜。
Appl Opt. 2008 Oct 20;47(30):5654-9. doi: 10.1364/ao.47.005654.
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Opt Lett. 2006 May 1;31(9):1211-3. doi: 10.1364/ol.31.001211.
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Digital in-line holographic microscopy.数字同轴全息显微镜。
Appl Opt. 2006 Feb 10;45(5):836-50. doi: 10.1364/ao.45.000836.
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Algorithm for reconstruction of digital holograms with adjustable magnification.具有可调放大率的数字全息图重建算法。
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