Tsaousis Konstantinos T, Karagiannidis Panagiotis G, Kopsachilis Nikolaos, Symeonidis Chrysanthos, Tsinopoulos Ioannis T, Karagkiozaki Varvara, Lamprogiannis Lampros P, Logothetidis Stergios
2nd Department of Ophthalmology, Medical School, Aristotle University of Thessaloniki, "Papageorgiou" General Hospital, 56429, Thessaloníki, Greece,
Int Ophthalmol. 2014 Jun;34(3):519-23. doi: 10.1007/s10792-013-9846-z. Epub 2013 Sep 15.
The purpose of the study was to appraise the effect of loading force magnitude on the determination of the elastic modulus of the anterior lens capsule through atomic force microscopy. Four human anterior lens capsules taken during phacoemulsification cataract surgery were studied, free of epithelial cells, with atomic force microscopy. For the experiment, five different indentation loading forces were applied to near areas of the specimen. Experimental data was exported and analyzed according to the Hertz model to obtain the Young's modulus with regards to the elastic behavior of the material. Force-distance curves were acquired by applying a load of 2, 5, 10, 20 and 30 nN. When examining the results it was evident that determination of Young's modulus of the anterior lens capsule is dependent on the loading force concerning the examined range. Loading forces of 10 and 20 nN led to results without significant difference (p > 0.05) and more reproducible (coefficients of variation 12.4 and 11.7 %, respectively).
本研究的目的是通过原子力显微镜评估加载力大小对前囊膜弹性模量测定的影响。对4例白内障超声乳化手术中获取的无上皮细胞的人眼前囊膜进行了原子力显微镜研究。实验中，对标本的临近区域施加了5种不同的压痕加载力。导出实验数据并根据赫兹模型进行分析，以获得材料弹性行为的杨氏模量。通过施加2、5、10、20和30 nN的载荷获取力-距离曲线。检查结果时发现，前囊膜杨氏模量的测定取决于所研究范围内的加载力。10和20 nN的加载力导致的结果无显著差异（p>0.05）且重复性更好（变异系数分别为12.4%和11.7%）。
