Efremov Yuri M, Bakhchieva Natalia A, Shavkuta Boris S, Frolova Anastasia A, Kotova Svetlana L, Novikov Ivan A, Akovantseva Anastasia A, Avetisov Konstantin S, Avetisov Sergei E, Timashev Peter S
Institute for Regenerative Medicine, Sechenov University, 8 Trubetskaya St., Moscow, 119991, Russia.
Research Institute of Eye Diseases, 11 Rossolimo St., Moscow, 119021, Russia.
J Mech Behav Biomed Mater. 2020 Dec;112:104081. doi: 10.1016/j.jmbbm.2020.104081. Epub 2020 Sep 15.
The purpose of this study is the mechanical characterization of the mid-to- old-age human anterior lens capsules (ALCs) obtained by capsulorhexis using Atomic Force Microscopy (AFM) and a nanoindenter at different spatial scales. The dependencies on the human age, presence or absence of pseudoexfoliation syndrome (PEX), and application of trypan blue staining during the surgery were analyzed. The measurements on both the anterior (AS) and epithelial (ES) sides of the ALC were conducted and the effect of cells present on the epithelial side was carefully accounted for. The ES of the ALC had a homogenous distribution of the Young's modulus over the surface as shown by the macroscale mapping with the nanoindenter and local AFM indentations, while the AS was more heterogeneous. Age-related changes were assessed in groups ranging from the mid-age (from 48 years) to old-age (up to 93 years). We found that the ES was always stiffer than the AS, and this difference decreased with age due to a gradual decrease in the Young's modulus of the ES and an increase in the modulus of the AS. No significant changes were found in the mechanical properties of ALCs of PEX patients versus the PEX-free group, as well as in the properties of the ALC with and without trypan blue staining.
本研究的目的是使用原子力显微镜（AFM）和纳米压痕仪在不同空间尺度上对通过撕囊术获得的中老年人类前囊膜（ALC）进行力学表征。分析了其与人类年龄、是否存在假性剥脱综合征（PEX）以及手术期间台盼蓝染色应用的相关性。对ALC的前侧（AS）和上皮侧（ES）均进行了测量，并仔细考虑了上皮侧存在的细胞的影响。如用纳米压痕仪进行的宏观尺度测绘和局部AFM压痕所示，ALC的ES在表面上杨氏模量分布均匀，而AS则更不均匀。在从中年（48岁起）到老年（93岁）的不同年龄组中评估了与年龄相关的变化。我们发现，ES总是比AS更硬，并且由于ES的杨氏模量逐渐降低和AS的模量增加，这种差异随着年龄的增长而减小。与无PEX组相比，PEX患者的ALC力学性能以及有或没有台盼蓝染色的ALC性能均未发现显著变化。
