Eidi Akram, Mortazavi Pejman, Behzadi Khodabakhsh, Rohani Ali Haeri, Safi Shahabeddin
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran,
Biol Trace Elem Res. 2013 Nov;155(2):267-75. doi: 10.1007/s12011-013-9784-7. Epub 2013 Sep 15.
The aim of the present study is to evaluate the protective effect of manganese chloride against carbon tetrachloride (CCl4)-induced liver injury in rats. Manganese chloride (0.001, 0.01, 0.05 and 0.1 g/kg bw) was administered intragastrically for 28 consecutive days to male CCl4-treated rats. The hepatoprotective activity was assessed using various biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), γ-glutamyltransferase (GGT) and superoxide dismutase (SOD). Histopathological changes in the liver of different groups were also studied. Administration of CCl4 increased the serum ALT, AST, ALP and GGT but decreased SOD levels in rats. Treatment with manganese chloride significantly attenuated these changes to nearly normal levels. The animals treated with manganese chloride have shown decreased necrotic zones and hepatocellular degeneration when compared to the liver exposed to CCl4 intoxication alone. Thus, the histopathological studies also supported the protective effect of manganese chloride. Therefore, the results of this study suggest that manganese chloride exerts hepatoprotection via promoting antioxidative properties against CCl4-induced oxidative liver damage.
本研究的目的是评估氯化锰对四氯化碳（CCl4）诱导的大鼠肝损伤的保护作用。将氯化锰（0.001、0.01、0.05和0.1 g/kg体重）连续28天经胃内给予雄性CCl4处理的大鼠。使用各种生化参数如丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、γ-谷氨酰转移酶（GGT）和超氧化物歧化酶（SOD）评估肝脏保护活性。还研究了不同组大鼠肝脏的组织病理学变化。给予CCl4会增加大鼠血清中的ALT、AST、ALP和GGT水平，但会降低SOD水平。用氯化锰治疗可显著减轻这些变化，使其接近正常水平。与仅暴露于CCl4中毒的肝脏相比，用氯化锰治疗的动物显示坏死区域和肝细胞变性减少。因此，组织病理学研究也支持了氯化锰的保护作用。因此，本研究结果表明，氯化锰通过促进抗氧化特性对CCl4诱导的氧化性肝损伤发挥肝脏保护作用。
