Xiao Liangpin, Liu Xianming, Zhong Runtao, Zhang Kaiqing, Zhang Xiaodi, Zhou Xiaomian, Lin Bingcheng, Du Yuguang
The Research Center of Lab on a Chip, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, P. R. China; School of Chemistry and Chemical Engineering of University of Chinese Academy of Science, Beijing, P. R. China.
Electrophoresis. 2013 Nov;34(20-21):3003-7. doi: 10.1002/elps.201300198. Epub 2013 Oct 7.
Three-dimensional (3D) paper-based microfluidics, which is featured with high performance and speedy determination, promise to carry out multistep sample pretreatment and orderly chemical reaction, which have been used for medical diagnosis, cell culture, environment determination, and so on with broad market prospect. However, there are some drawbacks in the existing fabrication methods for 3D paper-based microfluidics, such as, cumbersome and time-consuming device assembly; expensive and difficult process for manufacture; contamination caused by organic reagents from their fabrication process. Here, we present a simple printing-bookbinding method for mass fabricating 3D paper-based microfluidics. This approach involves two main steps: (i) wax-printing, (ii) bookbinding. We tested the delivery capability, diffusion rate, homogeneity and demonstrated the applicability of the device to chemical analysis by nitrite colorimetric assays. The described method is rapid (<30 s), cheap, easy to manipulate, and compatible with the flat stitching method that is common in a print house, making itself an ideal scheme for large-scale production of 3D paper-based microfluidics.
三维（3D）纸质微流控技术具有高性能和快速检测的特点，有望实现多步样品预处理和有序化学反应，已应用于医学诊断、细胞培养、环境检测等领域，具有广阔的市场前景。然而，现有的3D纸质微流控技术制造方法存在一些缺点，例如，设备组装繁琐且耗时；制造过程昂贵且困难；制造过程中有机试剂造成的污染。在此，我们提出一种用于大规模制造3D纸质微流控技术的简单印刷装订方法。该方法包括两个主要步骤：（i）蜡印，（ii）装订。我们测试了其输送能力、扩散速率、均匀性，并通过亚硝酸盐比色法证明了该设备在化学分析中的适用性。所描述的方法快速（<30秒）、廉价、易于操作，并且与印刷厂常见的平缝方法兼容，使其成为大规模生产3D纸质微流控技术的理想方案。
