Lu Yao, Shi Weiwei, Jiang Lei, Qin Jianhua, Lin Bingcheng
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, PR China.
Electrophoresis. 2009 May;30(9):1497-500. doi: 10.1002/elps.200800563.
Here we present a simple and low-cost production method to generate paper-based microfluidic devices with wax for portable bioassay. The wax patterning method we introduced here included three different ways: (i) painting with a wax pen, (ii) printing with an inkjet printer followed by painting with a wax pen, (iii) printing by a wax printer directly. The whole process was easy to operate and could be finished within 5-10 min without the use of a clean room, UV lamp, organic solvent, etc. Horse radish peroxidase, BSA and glucose assays were conducted to verify the performance of wax-patterned paper.
在此，我们展示了一种简单且低成本的生产方法，用于制造带有蜡的纸质微流控装置，以进行便携式生物测定。我们在此介绍的蜡图案化方法包括三种不同方式：（i）用蜡笔绘制，（ii）先用喷墨打印机打印，然后用蜡笔绘制，（iii）直接用蜡打印机打印。整个过程易于操作，无需使用洁净室、紫外线灯、有机溶剂等，5 - 10分钟内即可完成。进行了辣根过氧化物酶、牛血清白蛋白和葡萄糖测定，以验证蜡图案化纸张的性能。
