Sun Jinyu, Wu You, Xiao Dan, Lin Xia, Li Hui
College of Chemical Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
Luminescence. 2014 Aug;29(5):456-61. doi: 10.1002/bio.2571. Epub 2013 Aug 27.
An optimized and validated spectrofluorimetric method has been developed for the rapid determination of aluminum in absolute ethanol. The method is based on the chelation of aluminum and luteolin which results in a complex exhibiting an intense emission signal. The characterization of Al-luteolin complex was studied using ultraviolet-visible spectrometry, infrared spectrometry, fluorescence and mass spectrometry. The complex stoichiometry ratio of aluminum:luteolin was 1:2. The fluorescence of the complex was monitored at an emission wavelength of 545 nm with excitation at 518 nm. The linear concentration range was 6.5 × 10(-7) to 4.0 × 10(-5) M with a correlation coefficient of r = 0.998. The detection limit was 5.0 × 10(-7) M. The method was appropriately validated and yielded relative standard deviations of < 2.3% (n = 5), which was considered acceptable. The method was successfully applied in the determination of aluminum in river water, skin care products and pharmaceutical samples.
已开发出一种经过优化和验证的荧光分光光度法，用于快速测定无水乙醇中的铝。该方法基于铝与木犀草素的螯合作用，这会形成一种具有强烈发射信号的络合物。使用紫外可见光谱法、红外光谱法、荧光光谱法和质谱法对铝 - 木犀草素络合物进行了表征。铝与木犀草素的络合物化学计量比为1:2。在发射波长545 nm、激发波长518 nm下监测该络合物的荧光。线性浓度范围为6.5×10⁻⁷至4.0×10⁻⁵ M，相关系数r = 0.998。检测限为5.0×10⁻⁷ M。该方法经过适当验证，相对标准偏差<2.3%（n = 5），被认为是可接受的。该方法已成功应用于河水、护肤品和药物样品中铝的测定。
