Lee June Woo, Baek Seong Jin, Bae Woo Chul, Park Jeong Min, Kim Yong Seok
Department of Food Processing and Cooking, Kyungbuk College, Yeongjusi 750-712, Korea.
Mycobiology. 2006 Dec;34(4):230-5. doi: 10.4489/MYCO.2006.34.4.230. Epub 2006 Dec 31.
Fruiting bodies of Phellinus linteus were extracted by hot water and alkali methods. Sugar contents of PL-H (hot water extract) and PL-A (alkali water extract) were 81.1%, 37.4% and protein contents were 6.2%, 21.8%, respectively. Amino acid pattern showed that two extracts contained large amount of aspartic acid and alanine. Two extracts showed characteristic IR absorption pattern for glycosidic bond at 890 cm(-1). PL-H was divided two fractions by gel filtration chromatography and the molecular weights of each fraction were estimated to be about 10 kD and 225 kD, respectively and also PL-A was estimated 10 kD. Two extracts showed strong antitumor, immunomodulating and antioxidant activities, and were compared with commercialized glycopeptide anticancer drugs.
采用热水提取法和碱提取法提取了桑黄的子实体。热水提取物（PL-H）和碱水提取物（PL-A）的含糖量分别为81.1%、37.4%，蛋白质含量分别为6.2%、21.8%。氨基酸模式表明，两种提取物都含有大量的天冬氨酸和丙氨酸。两种提取物在890 cm(-1)处显示出糖苷键的特征红外吸收模式。通过凝胶过滤色谱法将PL-H分为两个组分，每个组分的分子量估计分别约为10 kD和225 kD，PL-A的分子量估计为10 kD。两种提取物均表现出较强的抗肿瘤、免疫调节和抗氧化活性，并与商业化的糖肽类抗癌药物进行了比较。
