Min Gyeong-Jin, Kang Hee-Wan
Graduate School of Future Convergence Technology, Hankyong National University, Ansung, Korea.
Department of Horticultural Biotechnology, Division of Biotechnology, Hankyong National University, Anseong, Korea.
Mycobiology. 2021 Apr 22;49(2):161-172. doi: 10.1080/12298093.2021.1892568. eCollection 2021.
strains were collected from different areas in Korea. Of them, the fast mycelial growing strains were artificially cultivated on the oak logs to produce fruiting body. The varieties, ASI26099 (Korea Sanghwang) and PBJS (Jangsoo Sanghwang) were grown under the same conditions as controls. Their cultivating characteristics including mycelial colonization, pinhead formation, and fruiting body formation rate were investigated on the logs. Basidiocarps of strains HN00K9, HN6036, and ASI26099 were concentrically zonate and shallowly sulcate, and dark chestnut showing typical characteristics of (synonyum: ), which is distinguishably different to PBJS. HN00K9 showed the highest yield of fruiting body among the mushroom strains. The β-glucan content in fruiting bodies of HN00K9 was 20% higher than those of other strains. Bioactive effects of polysaccharide samples from fruiting bodies of strains, HN00K9, HN6036, ASI26099, and PBJS were assessed on cell viability and cytokine (IL-6 and TNF-α) inhibition and finally on anticancer to different human cancer cells.
菌株从韩国不同地区采集。其中，生长迅速的菌丝体菌株在橡木原木上进行人工栽培以产生子实体。品种ASI26099（韩国桑黄）和PBJS（长寿桑黄）在相同条件下作为对照进行培养。在原木上研究了它们的培养特性，包括菌丝体定殖、原基形成和子实体形成率。菌株HN00K9、HN6036和ASI26099的担子果呈同心环带且有浅沟，颜色为深栗色，显示出（同义词： ）的典型特征，这与PBJS明显不同。在蘑菇菌株中，HN00K9的子实体产量最高。HN00K9子实体中的β-葡聚糖含量比其他菌株高20%。对菌株HN00K9、HN6036、ASI26099和PBJS子实体的多糖样品在细胞活力、细胞因子（IL-6和TNF-α）抑制以及最终对不同人类癌细胞的抗癌活性方面的生物活性作用进行了评估。
