Adaptation Physiology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands.
PLoS One. 2013 Sep 11;8(9):e72276. doi: 10.1371/journal.pone.0072276. eCollection 2013.
Specificity, antibody isotype distribution and levels of natural antibodies (NAb) may be potential informative parameters for immune mediated natural disease resistance, immune modulation, and maintenance of physiological homeostasis. A large proportion of mammalian NAb have affinity for or are directed against self-antigens; so called natural auto antibodies (N(A)Ab). In the present study we showed the presence and typed levels and isotypes (total immunoglobulins, IgG and IgM) of N(A)Ab in plasma binding the 'auto-antigen' complex chicken liver cell lysate (CLL) of one-year old chickens from different genotype and phenotype backgrounds by ELISA and quantitative Western blotting. Higher levels of N(A)Ab binding CLL were found in plasma from chickens genetically selected for high specific antibody responses. In all birds, extensive staining patterns of plasma antibodies binding CLL were found for all isotypes, with IgG binding the highest number of CLL antigens and also showing the highest variation in staining patterns between individuals. Patterns of IgM antibodies binding CLL appeared to be more similar in all lines. Significant differences of binding patterns of N(A)Ab (antigen fragments of CLL and staining intensity) were detected between the different chicken lines, and lines could be clustered on the basis of their auto-antibody profile. In addition, also individual differences within lines were found. The present results indicate that analysis of the levels and the N(A)Ab repertoire of poultry like in mammals could provide a new way of distinguishing differences of immune competence and immune maturation between individuals, and could provide tools to select birds for health traits, or optimize hygiene and husbandry procedures.
特异性、抗体同种型分布和天然抗体 (NAb) 的水平可能是免疫介导的天然疾病抗性、免疫调节和生理稳态维持的潜在信息参数。哺乳动物 NAb 的很大一部分对自身抗原具有亲和力或针对自身抗原；所谓的天然自身抗体 (N(A)Ab)。在本研究中，我们通过 ELISA 和定量 Western blot 显示了来自不同基因型和表型背景的 1 岁鸡血浆中存在的、针对“自身抗原”复合物鸡肝细胞裂解物 (CLL) 的 N(A)Ab 的水平和同种型 (总免疫球蛋白、IgG 和 IgM)。在遗传选择高特异性抗体反应的鸡的血浆中发现了更高水平的结合 CLL 的 N(A)Ab。在所有鸟类中，所有同种型的血浆抗体与 CLL 的结合都发现了广泛的染色模式，IgG 结合了 CLL 抗原的最高数量，并且在个体之间的染色模式变化也最高。IgM 抗体结合 CLL 的模式在所有品系中似乎更为相似。在不同的鸡系之间检测到 N(A)Ab（CLL 的抗原片段和染色强度）结合模式的显著差异，并且可以根据它们的自身抗体谱对鸡系进行聚类。此外，还发现了系内的个体差异。本研究结果表明，分析家禽如哺乳动物中的 NAb 水平和库可以提供一种新的方法来区分个体之间免疫能力和免疫成熟的差异，并为选择具有健康特征的鸟类或优化卫生和饲养程序提供工具。
