Giaquinta R T, Ort D R, Dilley R A
Biochemistry. 1975 Oct 7;14(20):4392-6. doi: 10.1021/bi00691a008.
Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfonate into membrane components. Previously it was established that electron flux from the photosystem II primary acceptor to plastoquinone is a necessary event for the occurrence of the diazonium-detected conformational change. These studies indicate that the release of hydrogen ions during photosystem II oxidation of the primary reductant is also a necessary event for the diazonium-detected conformational change. When iodide were substituted for water (or other proton-releasing donors) as the primary reductant of system II the conformational change did not occur even though a substantial rate of electron flow from the primary acceptor to plastoquinone occurred.
所呈现的数据表明，只有在片层膜经历构象变化后，依赖光系统II的氢离子积累和ATP合成才能发生。这种膜构象变化是通过电子传递依赖的重氮苯[35S]磺酸盐掺入膜成分来检测的。此前已确定，从光系统II初级受体到质体醌的电子通量是重氮检测到的构象变化发生的必要条件。这些研究表明，在光系统II氧化初级还原剂过程中氢离子的释放也是重氮检测到的构象变化的必要条件。当用碘化物代替水（或其他质子释放供体）作为系统II的初级还原剂时，即使从初级受体到质体醌有相当大的电子流速率，构象变化也不会发生。
