通过原位杂交法将肿瘤坏死因子信使核糖核酸定位于正常小鼠肠道上皮的潘氏细胞。
Tumor necrosis factor mRNA localized to Paneth cells of normal murine intestinal epithelium by in situ hybridization.
作者信息
Keshav S, Lawson L, Chung L P, Stein M, Perry V H, Gordon S
机构信息
Sir William Dunn School of Pathology, Oxford University, United Kingdom.
出版信息
J Exp Med. 1990 Jan 1;171(1):327-32. doi: 10.1084/jem.171.1.327.
Abstract
Paneth cells in normal murine small intestine contain TNF mRNA that is readily detectable by in situ hybridization, unlike resident macrophages in lamina propria, which are negative. Northern blot analysis of whole tissue shows the presence of mRNA that has the same electrophoretic mobility as TNF mRNA from activated macrophages. A low level of TNF bioactivity, but no immunoreactivity, was detected in normal small intestine, and TNF production in resting Paneth cells appears to be post-transcriptionally controlled. Typical leukocyte surface membrane markers were not found on Paneth cells, but were expressed by the surrounding lamina propria macrophages. Paneth cells are thus epithelial cells with leukocyte-like secretory potential that may be important in intestinal physiology and pathology.
摘要
正常小鼠小肠中的潘氏细胞含有可通过原位杂交轻易检测到的肿瘤坏死因子（TNF）信使核糖核酸（mRNA），这与固有层中呈阴性的常驻巨噬细胞不同。对全组织的Northern印迹分析显示存在与活化巨噬细胞的TNF mRNA具有相同电泳迁移率的mRNA。在正常小肠中检测到低水平的TNF生物活性，但未检测到免疫反应性，静息潘氏细胞中的TNF产生似乎受到转录后调控。在潘氏细胞上未发现典型的白细胞表面膜标志物，但周围固有层巨噬细胞表达这些标志物。因此，潘氏细胞是具有类似白细胞分泌潜能的上皮细胞，这可能在肠道生理和病理中起重要作用。
相似文献
1
Tumor necrosis factor mRNA localized to Paneth cells of normal murine intestinal epithelium by in situ hybridization.通过原位杂交法将肿瘤坏死因子信使核糖核酸定位于正常小鼠肠道上皮的潘氏细胞。
J Exp Med. 1990 Jan 1;171(1):327-32. doi: 10.1084/jem.171.1.327.
2
Cellular localization of tumor necrosis factor (TNF)-alpha transcripts in normal bowel and in necrotizing enterocolitis. TNF gene expression by Paneth cells, intestinal eosinophils, and macrophages.肿瘤坏死因子（TNF）-α转录本在正常肠道和坏死性小肠结肠炎中的细胞定位。潘氏细胞、肠道嗜酸性粒细胞和巨噬细胞的TNF基因表达。
Am J Pathol. 1993 Jun;142(6):1858-65.
3
Mast cells are an important cellular source of tumour necrosis factor alpha in human intestinal tissue.肥大细胞是人类肠道组织中肿瘤坏死因子α的重要细胞来源。
Gut. 1999 May;44(5):643-52. doi: 10.1136/gut.44.5.643.
4
Mouse endometrial tumor necrosis factor-alpha messenger ribonucleic acid and protein: localization and regulation by estradiol and progesterone.小鼠子宫内膜肿瘤坏死因子-α信使核糖核酸与蛋白：定位及雌二醇和孕酮对其的调节
Endocrinology. 1994 Dec;135(6):2780-9. doi: 10.1210/endo.135.6.7988471.
5
Regulation of tumor necrosis factor gene expression in colorectal adenocarcinoma: in vivo analysis by in situ hybridization.结直肠癌中肿瘤坏死因子基因表达的调控：原位杂交的体内分析
Proc Natl Acad Sci U S A. 1989 Jul;86(13):5064-8. doi: 10.1073/pnas.86.13.5064.
6
In situ hybridization analysis of macrophage-derived tumor necrosis factor and interleukin-1 mRNA.巨噬细胞源性肿瘤坏死因子和白细胞介素-1 mRNA的原位杂交分析
Lab Invest. 1988 Dec;59(6):809-16.
7
Butter feeding enhances TNF-alpha production from macrophages and lymphocyte adherence in murine small intestinal microvessels.喂食黄油可增强巨噬细胞产生肿瘤坏死因子-α以及小鼠小肠微血管中淋巴细胞的黏附。
J Gastroenterol Hepatol. 2007 Nov;22(11):1838-45. doi: 10.1111/j.1440-1746.2007.04905.x.
8
9
10
Detection of tumor necrosis factor-alpha protein and messenger RNA in human glial brain tumors: comparison of immunohistochemistry with in situ hybridization using molecular probes.
J Neurosurg. 1995 Aug;83(2):291-7. doi: 10.3171/jns.1995.83.2.0291.
引用本文的文献
1
Role of Tumor Necrosis Factor in Tuberculosis.肿瘤坏死因子在结核病中的作用。
Biomolecules. 2025 May 12;15(5):709. doi: 10.3390/biom15050709.
2
Epithelial TNF controls cell differentiation and CFTR activity to maintain intestinal mucin homeostasis.上皮细胞 TNF 控制细胞分化和 CFTR 活性以维持肠道粘蛋白的动态平衡。
J Clin Invest. 2023 Oct 16;133(20):e163591. doi: 10.1172/JCI163591.
3
Influence of on the small intestine and lactase enzyme activity.对小肠和乳糖酶活性的影响。
（你提供的原文“Influence of on the small intestine and lactase enzyme activity.”中“of”后面缺少具体内容，我根据常见语境补充完整后进行了翻译，你可根据实际情况调整。）
J Parasit Dis. 2022 Mar;46(1):243-253. doi: 10.1007/s12639-021-01442-6. Epub 2021 Aug 24.
4
Idebenone Protects against Spontaneous Chronic Murine Colitis by Alleviating Endoplasmic Reticulum Stress and Inflammatory Response.艾地苯醌通过减轻内质网应激和炎症反应来预防自发性慢性小鼠结肠炎。
Biomedicines. 2020 Sep 28;8(10):384. doi: 10.3390/biomedicines8100384.
5
Tumour Necrosis Factor Alpha in Intestinal Homeostasis and Gut Related Diseases.肿瘤坏死因子-α在肠道稳态和肠道相关疾病中的作用。
Int J Mol Sci. 2019 Apr 16;20(8):1887. doi: 10.3390/ijms20081887.
6
Paneth Cells during Viral Infection and Pathogenesis.潘氏细胞在病毒感染和发病机制中的作用。
Viruses. 2018 Apr 26;10(5):225. doi: 10.3390/v10050225.
7
Effects of endoplasmic reticulum stress on the expression of inflammatory cytokines in patients with ulcerative colitis.内质网应激对溃疡性结肠炎患者炎性细胞因子表达的影响。
World J Gastroenterol. 2016 Feb 21;22(7):2357-65. doi: 10.3748/wjg.v22.i7.2357.
8
Mechanisms of Microbe-Host Interaction in Crohn's Disease: Dysbiosis vs. Pathobiont Selection.克罗恩病中微生物与宿主相互作用的机制：生态失调与致病共生菌选择
Front Immunol. 2015 Nov 19;6:555. doi: 10.3389/fimmu.2015.00555. eCollection 2015.
9
Functional intersection of Human Defensin 5 with the TNF receptor pathway.人防御素 5 与 TNF 受体通路的功能交集。
FEBS Lett. 2014 May 21;588(10):1906-12. doi: 10.1016/j.febslet.2014.03.028. Epub 2014 Mar 26.
10
Innate IL-17-producing cells: the sentinels of the immune system.固有 IL-17 产生细胞：免疫系统的哨兵。
Nat Rev Immunol. 2010 Jul;10(7):479-89. doi: 10.1038/nri2800. Epub 2010 Jun 18.
本文引用的文献
1
The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia.通过抗原F4/80的免疫组织化学定位确定的小鼠单核吞噬细胞系统：与上皮相关的巨噬细胞。
Anat Rec. 1984 Nov;210(3):503-12. doi: 10.1002/ar.1092100311.
2
Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine. V. Unitarian Theory of the origin of the four epithelial cell types.小鼠小肠中四种主要上皮细胞类型的起源、分化和更新。V. 四种上皮细胞类型起源的统一理论。
Am J Anat. 1974 Dec;141(4):537-61. doi: 10.1002/aja.1001410407.
3
Localization of lysozyme activity in a Paneth cell granule fraction.溶菌酶活性在潘氏细胞颗粒组分中的定位。
Biochim Biophys Acta. 1967 May 16;139(1):204-7. doi: 10.1016/0005-2744(67)90136-2.
4
Ultrastructural immunocytochemical localization of lysozyme in the Paneth cells of man.溶菌酶在人潘氏细胞中的超微结构免疫细胞化学定位
J Histochem Cytochem. 1974 Jun;22(6):401-13. doi: 10.1177/22.6.401.
5
Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor.小鼠肿瘤坏死因子cDNA在大肠杆菌中的克隆与表达
Proc Natl Acad Sci U S A. 1985 Sep;82(18):6060-4. doi: 10.1073/pnas.82.18.6060.
6
Immunocytochemical localization of pancreatic carboxylic ester hydrolase in human paneth cells.胰腺羧酸酯水解酶在人潘氏细胞中的免疫细胞化学定位
Histochemistry. 1986;86(2):211-4. doi: 10.1007/BF00493390.
7
Biology of the macrophage.
J Cell Sci Suppl. 1986;4:267-86. doi: 10.1242/jcs.1986.supplement_4.16.
8
Cloning the human lysozyme cDNA: inverted Alu repeat in the mRNA and in situ hybridization for macrophages and Paneth cells.克隆人溶菌酶cDNA：mRNA中的反向Alu重复序列以及巨噬细胞和潘氏细胞的原位杂交
Proc Natl Acad Sci U S A. 1988 Sep;85(17):6227-31. doi: 10.1073/pnas.85.17.6227.
9
Immunohistochemical observations of lysozyme in the Paneth cells of specific-pathogen-free and germ-free mice.无特定病原体小鼠和无菌小鼠潘氏细胞中溶菌酶的免疫组织化学观察
Acta Histochem. 1988;83(2):185-8. doi: 10.1016/S0065-1281(88)80055-2.
10
Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium.隐窝素（一种在小鼠小肠隐窝上皮中的皮质抑素/防御素前体mRNA）的发育调控。
J Cell Biol. 1989 May;108(5):1687-95. doi: 10.1083/jcb.108.5.1687.
Zotero 插件