Basuony Gehad A, M A Basyoni Maha, Negm Mohamed Sherif Ismail, Mostafa Eman Ali Mohamed, El-Wakil Eman Sayed, Shemis Mohamed A, Gouda Abdullah E, Saftawy Enas A El
Parasitology Department, Kasr Al-Ainy School of Medicine, Cairo University, Cairo, Egypt.
Pathology Department, Kasr Al-Ainy School of Medicine, Cairo University, Cairo, Egypt.
J Parasit Dis. 2022 Mar;46(1):243-253. doi: 10.1007/s12639-021-01442-6. Epub 2021 Aug 24.
is a cosmopolitan protozoan that has been associated with several gastrointestinal disturbances involving lactose intolerance. However, the underlying pathogenic factors remain indistinct. 20 Swiss albino mice were utilized and assembled into four groups, each of five mice: group-I: received neither infection nor lactose (healthy control), group-II: received a single dose of 10,000 cysts of and lactose diets in a dose of 12.5 g/day/mouse for 7 consecutive days starting from day 14 p.i., group-III: non-infected mice with oral doses of lactose (12.5 g/day/mouse) for 7 consecutive days (positive control), group-IV: infected mice on lactose free diet (negative control). We investigated the histopathological changes using H&E stain.s Also, lactase enzyme activity was measured using spectrophotometry and the production of TNF-α and apoptotic events were explored via immunohistochemistry and compared in the small intestine of all groups. The active inflammatory changes in the infected animals were moderate in the form of loss of villous architecture, increased ILC (-value > 0.001) besides scattered forms of the parasite as compared to non-infected mice. There was a reduction in lactase enzyme activity p.i. The TNF-α levels were induced p.i. as compared to non-infected mice (-value > 0.001). The expression of Bax protein was upgraded, while Bcl-2 expression decreased significantly with a reverse in Bax/Bcl2 ratio in infected animals. infection appears to humble lactase enzyme activity via the induction of apoptosis in the epithelial cells of the small intestinal brush border in a TNF-α associative pathway.
是一种世界性的原生动物，与多种涉及乳糖不耐受的胃肠道紊乱有关。然而，潜在的致病因素仍不明确。使用了20只瑞士白化小鼠并将其分为四组，每组五只小鼠：第一组：既未感染也未摄入乳糖（健康对照）；第二组：从感染后第14天开始，接受单剂量10000个囊肿，并以12.5克/天/只的剂量连续7天给予乳糖饮食；第三组：口服乳糖（12.5克/天/只）连续7天的未感染小鼠（阳性对照）；第四组：无乳糖饮食的感染小鼠（阴性对照）。我们使用苏木精-伊红染色研究了组织病理学变化。此外，使用分光光度法测量乳糖酶活性，并通过免疫组织化学探究肿瘤坏死因子-α（TNF-α）的产生和凋亡事件，并在所有组的小肠中进行比较。与未感染小鼠相比，感染动物的活跃炎症变化为中度，表现为绒毛结构丧失、固有层淋巴细胞增加（P值>0.001）以及散在形式的寄生虫。感染后乳糖酶活性降低。与未感染小鼠相比，感染后TNF-α水平升高（P值>0.001）。在感染动物中，Bax蛋白表达上调，而Bcl-2表达显著下降，Bax/Bcl2比值逆转。感染似乎通过TNF-α相关途径诱导小肠刷状缘上皮细胞凋亡，从而降低乳糖酶活性。
