Ferreira O C, Garcia-Pardo A, Bianco C
Lindsley F. Kimball Research Institute, New York Blood Center, New York 10021.
J Exp Med. 1990 Jan 1;171(1):351-6. doi: 10.1084/jem.171.1.351.
U937 cells attach to the RGDS-containing 80-kD fragment of fibronectin (Fn). The present report examined whether these cells recognize other domains of Fn. U937 cells attach to a 38-kD fragment derived from the A chain of Fn, which includes the Hep II domain and most of the alternatively spliced IIICS region. U937 did not bind to a 58-kD fragment derived from the B chain (which lacks IIICS) and has the Hep II site. They also did not bind to a 31-kD COOH-terminal fibrin-binding fragment or to a 29-kD fragment containing the Hep I domain. Cell adhesion to the 38-kD fragment was not inhibited by the 80-kD fragment, by GRGDSPC synthetic peptides, or by a mAb directed to the RGDS-containing domain of Fn. Attachment was completely inhibited by the 38-kD fragment and by the synthetic peptide CS-1, comprising the first 25 amino acid residues of IIICS. These results indicate that U937 cells interact with two sites of Fn, the RGDS-containing region, and the IIICS region.
U937细胞可附着于含RGDS的纤连蛋白（Fn）80-kD片段。本报告研究了这些细胞是否能识别Fn的其他结构域。U937细胞可附着于源自Fn A链的38-kD片段，该片段包含Hep II结构域和大部分可变剪接的IIICS区域。U937细胞不与源自B链（缺乏IIICS）且具有Hep II位点的58-kD片段结合。它们也不与31-kD的COOH末端纤维蛋白结合片段或含有Hep I结构域的29-kD片段结合。细胞对38-kD片段的黏附不受80-kD片段、GRGDSPC合成肽或针对Fn含RGDS结构域的单克隆抗体的抑制。黏附被38-kD片段和包含IIICS前25个氨基酸残基的合成肽CS-1完全抑制。这些结果表明，U937细胞与Fn的两个位点相互作用，即含RGDS的区域和IIICS区域。
