通过共价固定法固定在甲基丙烯酸羟烷基酯凝胶上的胃蛋白酶：制备与表征
Pepsin immobilized by covalent fixation to hydroxyalkyl methacrylate gels: preparation and characterization.
作者信息
Valentová O, Turková J, Lapka R, Zima J, Coupek J
出版信息
Biochim Biophys Acta. 1975 Sep 22;403(1):192-6. doi: 10.1016/0005-2744(75)90021-2.
PMID:240427
Abstract
Insoluble active derivatives of pepsin (EC 3.4.23.1) were prepared by covalent binding of this enzyme to hydroxyalkyl methacrylate gels modified with 1,6-diaminohexane or epsilon-aminocaproic acid in an acid medium by means of water-soluble carbodiimide. The amount of attached enzyme, its proteolytic activity, pH activity curves of the preparations obtained and the time and pH dependence of their stability were determined.
摘要
通过在酸性介质中利用水溶性碳二亚胺，将胃蛋白酶（EC 3.4.23.1）与用1,6 - 二氨基己烷或ε-氨基己酸修饰的甲基丙烯酸羟烷基酯凝胶共价结合，制备了胃蛋白酶的不溶性活性衍生物。测定了附着酶的量、其蛋白水解活性、所得制剂的pH活性曲线以及它们稳定性的时间和pH依赖性。
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