Xie Le-Yun, Zhong Li-Li, Zhang Bing, Duan Zhao-Jun, Xie Zhi-Ping, Gao Han-Chun, Chen Qian-Qian, Deng Zhong-Hua, Lin Lin, Gao Xiao-Qian, Xiao Ni-Guang
The First Affiliated Hospital of Hunan Normal University, Changsha 410000, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013 Apr;27(2):95-7.
To investigate the prevalence of viral pathogen in children with severe pneumonia in Hunan.
Bronchoalveolar lavage fluid [BALF] were collected from 122 hospitalized children with severe pneumonia in People's Hospital of Hunan province from January 2011 to December 2011. Nested- or reverse transcription Polymerase chain reaction (PCR or RT-PCR) was used to screen Adenovirus (ADV), Human Bocavirus (HBoV), Parainfluenzaviruses1-4 (PIV1-4), Human Respiratory Syneytial virus (RSV), Influenza virus A (IFVA), Influenza virus B (IFVB), Human Rhinovirus(HRV), Human Metapneumovirus (HMPV), human coronaviruses NL63 and HKU1 (HCoV-NL63, HCoV- HKU1).
Among the 122 bronchoalveolar lavage fluid, viral agents were detected in 60 samples(49.1%), among which ADV (40.98%) was the most common virus, followed by RSV (7.37%) and HBoV (7.37%). Two viruses were detected in 21 individual (35%) samples, of which 20 were dual positive for ADV (40%).
ADV is the most frequently detected viral etiology of severe pneumonia in children in Hunan during this year. And its Coinfection with other respiratory viruses was common.
调查湖南重症肺炎患儿中病毒病原体的流行情况。
收集2011年1月至2011年12月在湖南省人民医院住院的122例重症肺炎患儿的支气管肺泡灌洗液（BALF）。采用巢式或逆转录聚合酶链反应（PCR或RT-PCR）检测腺病毒（ADV）、人博卡病毒（HBoV）、副流感病毒1-4型（PIV1-4）、人呼吸道合胞病毒（RSV）、甲型流感病毒（IFVA）、乙型流感病毒（IFVB）、人鼻病毒（HRV）、人偏肺病毒（HMPV）、人冠状病毒NL63和HKU1（HCoV-NL63、HCoV-HKU1）。
在122份支气管肺泡灌洗液中，60份样本（49.1%）检测到病毒病原体，其中ADV（40.98%）是最常见的病毒，其次是RSV（7.37%）和HBoV（7.37%）。21份个体样本（35%）检测到两种病毒，其中20份为ADV双重阳性（40%）。
ADV是该年度湖南儿童重症肺炎中最常检测到的病毒病因，且其与其他呼吸道病毒的合并感染很常见。
