Xiao Ni-Guang, Zhang Bing, Duan Zhao-Jun, Xie Zhi-Ping, Zhou Qiong-Hua, Zhong Li-Li, Gao Han-Chun, Ding Xiao-Fang, Zeng Sai-Zhen, Huang Han, Hou Yun-De
Department of Pediatrics, People's Hospital of Hunan Province, Changsha 410005, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2012 Jan;14(1):28-32.
To explore the viral etiology of acute low respiratory tract infection (ALRTI) among hospitalized children in Changsha of Hunan Province of China.
Nasopharyngeal aspirates were collected from 1165 hospitalized children with ALRTI in Changsha from September 2007 to August 2008. Respiratory syncytin virus (RSV), human rhinovirus (HRV), influenza virus A (IFVA), influenza virus B (IFVB), parainfluenza 1-3 (PIV 1-3), human metapneumovirus (hMPV), human coronaviruses NL63 (HCoV-NL63), and human coronaviruses HKU1 (HCoV-HKU1) were detected by reverse transcription polymerase chain reaction (RT-PCR). Adenovirus (ADV) and human bocavirus (HBoV) were detected by standard polymerase chain reaction (PCR). WU polyomaviruses (WUPyV) and KI polyomaviruses(KIPyV) were detected by nested PCR. The positive samples further underwent genetic sequencing.
Among the 1165 nasopharyngeal aspirates, viruses were detected in 871 samples (74.76%), among which RSV (27.03%) was the most common virus, followed by HRV (17.33%), PIV3 (13.73%), HBoV (8.67%) and hMPV (6.52%). The overall positive rate of viral detection showed no significant differences between males and females (X2=2.241, P=0.134), whereas the positive rates of PIV3, hMPV, and HBoV in males were higher than in females. The positive rate of viral detection showed significant differences among different age groups (X2=10.934, P=0.027), and the highest positive rate was noted in the age group of 6 months to 1 year. Furthermore, the overall positive rate of viral detection showed a significant difference in term of seasonal distribution, with a peak prevalence in winter.
Virues predominate in the etiology of pediatric ALRTI in Changsha, and RSV, HRV and PIV3 are the main viruses for ALRTI. HBoV and hMPV have become increasingly important. Viral infection-associated ALRTI shows a prevail in the age group of 6 months to 1 year as well as in winter.
探讨中国湖南省长沙市住院儿童急性下呼吸道感染（ALRTI）的病毒病因。
收集2007年9月至2008年8月在长沙住院的1165例ALRTI儿童的鼻咽抽吸物。采用逆转录聚合酶链反应（RT-PCR）检测呼吸道合胞病毒（RSV）、人鼻病毒（HRV）、甲型流感病毒（IFVA）、乙型流感病毒（IFVB）、副流感病毒1-3型（PIV 1-3）、人偏肺病毒（hMPV）、人冠状病毒NL63（HCoV-NL63）和人冠状病毒HKU1（HCoV-HKU1）。采用标准聚合酶链反应（PCR）检测腺病毒（ADV）和人博卡病毒（HBoV）。采用巢式PCR检测WU多瘤病毒（WUPyV）和KI多瘤病毒（KIPyV）。对阳性样本进一步进行基因测序。
在1165份鼻咽抽吸物中，871份样本（74.76%）检测到病毒，其中RSV（27.03%）是最常见的病毒，其次是HRV（17.33%）、PIV3（13.73%）、HBoV（8.67%）和hMPV（6.52%）。病毒检测总体阳性率在男性和女性之间无显著差异（X2=2.241，P=0.134），而男性中PIV3、hMPV和HBoV的阳性率高于女性。病毒检测阳性率在不同年龄组之间存在显著差异（X2=10.934，P=0.027），6个月至1岁年龄组阳性率最高。此外，病毒检测总体阳性率在季节分布方面存在显著差异，冬季患病率最高。
病毒在长沙儿童ALRTI病因中占主导地位，RSV、HRV和PIV3是ALRTI的主要病毒。HBoV和hMPV的重要性日益增加。病毒感染相关的ALRTI在6个月至1岁年龄组以及冬季较为普遍。
