Stepniewski Jacek, Józkowicz Alicja, Dulak Józef
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa St., 30-387 Cracow, Poland.
Postepy Biochem. 2013;59(2):157-63.
Reprogramming of somatic cells with defined transcription factors which leads to the acquisition of pluripotent phenotype requires many modifications in gene expression profile, metabolism and chromatin structure. MicroRNAs, a short non-coding RNA that inhibit translation of recognized mRNA sequences play an important role in this process. Because of the pleiotropism of their action microRNAs regulate subsequent steps in cellular reprogramming, among others activation of p53, mesenchymal-to-epithelial transition and development of embryonic gene expression profile. Further studies on the function of microRNA in reprogramming can increase our understandbing of generation, maintenance and differentiation of induced pluripotent stem cells.
利用特定转录因子对体细胞进行重编程，使其获得多能表型，这需要基因表达谱、代谢和染色质结构发生许多改变。微小RNA是一种短的非编码RNA，可抑制已识别的mRNA序列的翻译，在这一过程中发挥重要作用。由于其作用具有多效性，微小RNA可调节细胞重编程的后续步骤，其中包括p53的激活、间充质向上皮转化以及胚胎基因表达谱的形成。对微小RNA在重编程中功能的进一步研究，可增进我们对诱导多能干细胞的产生、维持和分化的理解。
