Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
Anal Biochem. 2013 Dec 15;443(2):156-65. doi: 10.1016/j.ab.2013.09.008. Epub 2013 Sep 14.
Actin is a multifunctional eukaryotic protein with a globular monomer form that polymerizes into a thin, linear microfilament in cells. Through interactions with various actin-binding proteins (ABPs), actin plays an active role in many cellular processes, such as cell motility and structure. Microscopy techniques are powerful tools for determining the role and mechanism of actin-ABP interactions in these processes. In this article, we describe the basic concepts of fluorescent speckle microscopy, total internal reflection fluorescence microscopy, atomic force microscopy, and cryoelectron microscopy and review recent studies that utilize these techniques to visualize the binding of actin with ABPs.
肌动蛋白是一种多功能的真核生物蛋白，具有球形单体形式，在细胞中聚合成长而细的线性微丝。通过与各种肌动蛋白结合蛋白（ABP）相互作用，肌动蛋白在许多细胞过程中发挥着积极的作用，如细胞运动和结构。显微镜技术是确定肌动蛋白-ABP 相互作用在这些过程中的作用和机制的有力工具。在本文中，我们描述了荧光斑点显微镜、全内反射荧光显微镜、原子力显微镜和冷冻电子显微镜的基本概念，并回顾了利用这些技术来可视化肌动蛋白与 ABP 结合的最新研究。
