Laboratory Animal Center, Academy of Military Medical Science, Beijing, 100071, China.
Immunogenetics. 2013 Dec;65(12):851-9. doi: 10.1007/s00251-013-0735-4. Epub 2013 Sep 18.
Tibetan macaques (Macaca thibetana), stump-tailed macaques (M. arctoides), Assamese macaques (M. assamensis), and northern pig-tailed macaques (M. leonina) are four major species of Macaca in China. In order to effectively use these species in biomedical research, thorough investigations of their MHC immunogenetics are required. In this study, we identified MHC class I sequences using cDNA cloning and sequencing on a cohort of six M. thibetana, three M. arctoides, three M. assamensis, and three M. leonina derived from Sichuan and Yunnan provinces of China. Eighty new alleles were identified, including 26 MHC-A alleles, 46 MHC-B alleles, and 8 MHC-I alleles. Among them, Math-A1126:01, Math-B190:01, Math-B191:01, Math-B192:01, Maar-A1127:01, Maar-A1129:01, and Maas-A1*128:01 represent lineages that had not been reported earlier in Macaca. Phylogenetic analyses show that no obvious separation of lineages among these species of Macaca. This study provides important information about the MHC immunogenetics for the four major species of Chinese macaques and adds value to these species as model organisms in biomedical research.
藏酋猴（Macaca thibetana）、短尾猴（M. arctoides）、阿萨姆猴（M. assamensis）和豚尾猴（M. leonina）是中国的四大猕猴属物种。为了在生物医学研究中有效利用这些物种，需要对其 MHC 免疫遗传学进行深入研究。在这项研究中，我们使用 cDNA 克隆和测序方法，对来自中国四川和云南两省的六只藏酋猴、三只短尾猴、三只阿萨姆猴和三只豚尾猴进行了 MHC 类 I 序列鉴定。共鉴定出 80 个新等位基因，包括 26 个 MHC-A 等位基因、46 个 MHC-B 等位基因和 8 个 MHC-I 等位基因。其中，Math-A1126:01、Math-B190:01、Math-B191:01、Math-B192:01、Maar-A1127:01、Maar-A1129:01 和 Maas-A1*128:01 代表猕猴属中以前未报道过的谱系。系统发育分析表明，这些猕猴属物种之间没有明显的谱系分离。本研究为中国四大猕猴属物种的 MHC 免疫遗传学提供了重要信息，并为这些物种作为生物医学研究中的模型动物增添了价值。
